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Invitrogen™ HPV Type 16 E7 Monoclonal Antibody (8C9), 50 µg
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Specifications:
| Application | Western Blot, immunocytochemistry, Immunohistochemistry, Immunoprecipitation (IP) | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ HPV Type 16 E7 Monoclonal Antibody (Clone 8C9) is a highly specific mouse IgG1 monoclonal antibody raised against a bacterially derived fusion protein containing the open reading frame (ORF) of the human papillomavirus (HPV) type 16 E7 early protein. The E7 protein is a well-known viral oncoprotein involved in the inactivation of tumor suppressor pRb and plays a critical role in HPV-mediated cellular transformation.
This antibody is designed for research applications including Western blotting, immunoprecipitation, immunohistochemistry (paraffin-embedded), and immunocytochemistry, providing reliable detection of HPV16 E7 expression in cell lines and tissue samples.
Applications & Tested Dilutions
| Application | Dilution | Published Uses |
|---|---|---|
| Western Blot (WB) | 1:100 | 19 publications |
| Immunoprecipitation (IP) | 1:10 | 2 publications |
| Immunohistochemistry (Paraffin) | 1:2 | Noted in datasheet |
| Immunocytochemistry (ICC/IF) | Not determined | 6 publications |
Key Features and Benefits
- High Specificity: Selectively binds to HPV Type 16 E7 with no cross-reactivity to HPV16-E2, E4, E6, HPV6/18 E7, or HeLa/HT-3/C-33A cell lines.
- Clone 8C9: Monoclonal antibody targeting conserved regions of the E7 protein, recognizing the 15 kDa protein expressed in CaSki (HPV16+) cells.
- Multiple Validations: Verified across 8 published figures and over 28 peer-reviewed publications.
- Lyophilized Format: Reconstitutes easily in 1 mL of deionized or distilled water to 50 µg/mL.
- HPLC-Purified: Ensures consistent performance and low background.
- Supplied with Preservatives: Contains PBS with 1% BSA and 0.1% sodium azide for stability.
Molecular Background
HPV16 E7 is a 98-amino acid phosphoprotein (~15 kDa) with structural and functional homology to adenoviral E1A proteins. It contains two conserved domains:
- CR1/CR2 domains (amino acids 1–37): Involved in cellular transformation.
-
LXCXE motif (residues 22–26): Mediates binding to tumor suppressor protein pRb.
This interaction deregulates the cell cycle, contributing to oncogenesis in HPV-related cancers.
Specifications
| Property | Detail |
|---|---|
| Clone | 8C9 |
| Type | Monoclonal antibody |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Reactivity | Virus (HPV Type 16) |
| Storage Buffer | PBS + 1% BSA + 0.1% sodium azide |
| Storage Conditions | 4 °C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| Reconstitution | Add 1 mL of distilled or deionized water |
| RRID | AB_2533057 |
The Invitrogen™ HPV Type 16 E7 Monoclonal Antibody (8C9) is a trusted and extensively validated tool for the detection of HPV16 E7 oncoprotein in virology, oncology, and vaccine development research. Its specificity for HPV16-positive samples and exclusion of cross-reactivity with other HPV types or cell lines make it an ideal choice for researchers investigating viral oncogenesis, tumor suppressor interactions, and HPV-mediated transformation.