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Thermo Scientific™ Brij™-35, 30% Solution, 950 mL
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Specifications:
| Application | Protein Purification | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Brij™-35, 30% Solution is a high-purity, stabilized nonionic detergent widely used in protein research and chromatographic applications. Supplied as a 30% (w/w) aqueous solution, this reagent-grade surfactant is ideal for cell lysis protocols, membrane protein extraction, and HPLC mobile phase formulations. Brij™-35 provides gentle solubilization of proteins and lipids without denaturing their native structure, making it an indispensable reagent in biochemical workflows.
Key Features
- High-Purity Detergent Solution – Stabilized 30% (w/w) Brij-35 in ultrapure water
- Nonionic Surfactant – Ideal for preserving protein function during lysis and solubilization
- Versatile Use – Compatible with a wide range of biochemical, proteomic, and HPLC methods
- Convenient Format – Ready-to-use liquid formulation simplifies dilution and handling
- Stable Performance – Clear, colorless viscous liquid, free from foreign materials
Applications
- Protein Research – Cell membrane solubilization, membrane protein isolation, and enzyme assays
- Chromatography – Surfactant additive for reversed-phase and ion-exchange HPLC mobile phases
- Biochemical Buffer Formulation – Included in detergent-based lysis buffers for gentle protein extraction
- Micelle Formation Studies – Useful for research involving micelle-based solubilization or encapsulation
Chemical and Physical Properties
| Property | Specification |
|---|---|
| Molecular Weight | 1225 g/mol |
| Detergent Class | Nonionic |
| Concentration | 27.0 – 30.0% (w/w) |
| Critical Micelle Conc. | 0.09 mM (0.011%, w/v) |
| Aggregation Number | 40 |
| Micelle Mol. Weight | 49,000 g/mol |
| Cloud Point | >100 °C |
| Dialyzable | No |
| Appearance | Clear, colorless viscous liquid |
| Form | Liquid |
| Storage Conditions | Ambient temperature (Room temp) |
| Reagent Type | Detergent Solution |
| Product Line | Brij™, Surfact-Amps™ |
| Unit Size | 950 mL |
Thermo Scientific™ Brij™-35, 30% Solution delivers a consistent and gentle nonionic detergent solution for advanced biochemical and chromatographic applications. Its ready-to-use format, high purity, and stable performance make it an essential reagent for research laboratories focused on protein science and analytical separations.