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High-Purity Quantitative Standard for HPLC Analysis of Protein Hydrolysates
The Thermo Scientific™ Pierce™ Amino Acid Standard H is a certified quantitative mixture of 18 L-form amino acids, each supplied at 2.5 μmol/mL in 0.1N HCl (except cystine at 1.25 μmol/mL), specifically formulated for high-accuracy amino acid composition analysis of peptides and proteins by HPLC.
Pre-aliquoted in sealed 1 mL glass ampules to maintain stability and prevent contamination, this ready-to-use standard is ideal for HPLC calibration, protein hydrolysate analysis, and qualitative/quantitative amino acid profiling. All amino acid concentrations are validated by conventional amino acid analysis methods to ensure traceability, reproducibility, and analytical confidence.
Amino Acid Composition
| Amino Acid | Concentration (μmol/mL) | Solvent |
|---|---|---|
| L-Alanine | 2.5 | 0.1N HCl |
| L-Arginine | 2.5 | 0.1N HCl |
| L-Aspartic Acid | 2.5 | 0.1N HCl |
| L-Cystine | 1.25 | 0.1N HCl |
| L-Glutamic Acid | 2.5 | 0.1N HCl |
| Glycine | 2.5 | 0.1N HCl |
| L-Histidine | 2.5 | 0.1N HCl |
| L-Isoleucine | 2.5 | 0.1N HCl |
| L-Leucine | 2.5 | 0.1N HCl |
| L-Lysine·HCl | 2.5 | 0.1N HCl |
| L-Methionine | 2.5 | 0.1N HCl |
| L-Phenylalanine | 2.5 | 0.1N HCl |
| L-Proline | 2.5 | 0.1N HCl |
| L-Serine | 2.5 | 0.1N HCl |
| L-Threonine | 2.5 | 0.1N HCl |
| L-Tyrosine | 2.5 | 0.1N HCl |
| L-Valine | 2.5 | 0.1N HCl |
| Ammonia (NH₄⁺ source) | 2.5 | 0.1N HCl |
Key Features & Benefits
- Quantitative Calibration Standard – Precisely formulated with verified concentrations for reproducible results.
- Ready-to-Use – Pre-measured in 1 mL sealed ampules, eliminating the need for preparation.
- Stable Formulation – Dissolved in 0.1N HCl for long shelf life; stable between –20°C and 8°C.
- L-Amino Acids Only – Ensures assay standardization for microbial, biochemical, and chromatographic applications.
- Certified Quality – Concentrations validated by amino acid analysis for traceable, reproducible performance.
Applications
- HPLC calibration of amino acid analyzers
- Quantitative analysis of protein hydrolysates
- Verification of peptide and protein compositions
- Microbiological media testing
- Standardization of amino acid assays in biotech and pharmaceutical research
Technical Specifications
| Attribute | Details |
|---|---|
| Quantity | 10 × 1 mL sealed ampules |
| Concentration | 2.5 μmol/mL for each amino acid (1.25 μmol/mL for cystine) |
| Form | Liquid |
| Solvent | 0.1N HCl |
| Amino Acids Included | 18 L-form amino acids |
| Shelf Life (Unopened) | Indefinite when stored frozen |
| Shelf Life (After Opening) | 6 months (store frozen between uses) |
| Storage Conditions | –20°C to 8°C |
| Regulatory Use | For research use only |
Contents
- 10 × 1 mL glass ampules of amino acid standard solution
- High-quality packaging to ensure sterility and stability
- Compatible with standard amino acid analyzers and HPLC systems
Handling & Usage Notes
- Shake well after thawing to ensure homogeneity.
- Use ampule breakers or other safety tools when opening glass ampules.
- Avoid repeated freeze–thaw cycles.
The Thermo Scientific™ Pierce™ Amino Acid Standard H is a trusted, high-purity quantitative standard for amino acid analysis in proteomics, biotechnology, and quality control laboratories. Its precise formulation, long-term stability, and certified concentrations provide researchers with confidence in achieving accurate, sensitive, and reproducible amino acid composition analysis using HPLC or amino acid analyzers.
Application Note: Amino Acid Analysis of Mammalian Cell Culture Medium by Liquid Chromatography with UV and Fluorescence Detection and Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate
High-Purity Quantitative Standard for HPLC Analysis of Protein Hydrolysates
The Thermo Scientific™ Pierce™ Amino Acid Standard H is a certified quantitative mixture of 18 L-form amino acids, each supplied at 2.5 μmol/mL in 0.1N HCl (except cystine at 1.25 μmol/mL), specifically formulated for high-accuracy amino acid composition analysis of peptides and proteins by HPLC.
Pre-aliquoted in sealed 1 mL glass ampules to maintain stability and prevent contamination, this ready-to-use standard is ideal for HPLC calibration, protein hydrolysate analysis, and qualitative/quantitative amino acid profiling. All amino acid concentrations are validated by conventional amino acid analysis methods to ensure traceability, reproducibility, and analytical confidence.
Amino Acid Composition
| Amino Acid | Concentration (μmol/mL) | Solvent |
|---|---|---|
| L-Alanine | 2.5 | 0.1N HCl |
| L-Arginine | 2.5 | 0.1N HCl |
| L-Aspartic Acid | 2.5 | 0.1N HCl |
| L-Cystine | 1.25 | 0.1N HCl |
| L-Glutamic Acid | 2.5 | 0.1N HCl |
| Glycine | 2.5 | 0.1N HCl |
| L-Histidine | 2.5 | 0.1N HCl |
| L-Isoleucine | 2.5 | 0.1N HCl |
| L-Leucine | 2.5 | 0.1N HCl |
| L-Lysine·HCl | 2.5 | 0.1N HCl |
| L-Methionine | 2.5 | 0.1N HCl |
| L-Phenylalanine | 2.5 | 0.1N HCl |
| L-Proline | 2.5 | 0.1N HCl |
| L-Serine | 2.5 | 0.1N HCl |
| L-Threonine | 2.5 | 0.1N HCl |
| L-Tyrosine | 2.5 | 0.1N HCl |
| L-Valine | 2.5 | 0.1N HCl |
| Ammonia (NH₄⁺ source) | 2.5 | 0.1N HCl |
Key Features & Benefits
- Quantitative Calibration Standard – Precisely formulated with verified concentrations for reproducible results.
- Ready-to-Use – Pre-measured in 1 mL sealed ampules, eliminating the need for preparation.
- Stable Formulation – Dissolved in 0.1N HCl for long shelf life; stable between –20°C and 8°C.
- L-Amino Acids Only – Ensures assay standardization for microbial, biochemical, and chromatographic applications.
- Certified Quality – Concentrations validated by amino acid analysis for traceable, reproducible performance.
Applications
- HPLC calibration of amino acid analyzers
- Quantitative analysis of protein hydrolysates
- Verification of peptide and protein compositions
- Microbiological media testing
- Standardization of amino acid assays in biotech and pharmaceutical research
Technical Specifications
| Attribute | Details |
|---|---|
| Quantity | 10 × 1 mL sealed ampules |
| Concentration | 2.5 μmol/mL for each amino acid (1.25 μmol/mL for cystine) |
| Form | Liquid |
| Solvent | 0.1N HCl |
| Amino Acids Included | 18 L-form amino acids |
| Shelf Life (Unopened) | Indefinite when stored frozen |
| Shelf Life (After Opening) | 6 months (store frozen between uses) |
| Storage Conditions | –20°C to 8°C |
| Regulatory Use | For research use only |
Contents
- 10 × 1 mL glass ampules of amino acid standard solution
- High-quality packaging to ensure sterility and stability
- Compatible with standard amino acid analyzers and HPLC systems
Handling & Usage Notes
- Shake well after thawing to ensure homogeneity.
- Use ampule breakers or other safety tools when opening glass ampules.
- Avoid repeated freeze–thaw cycles.
The Thermo Scientific™ Pierce™ Amino Acid Standard H is a trusted, high-purity quantitative standard for amino acid analysis in proteomics, biotechnology, and quality control laboratories. Its precise formulation, long-term stability, and certified concentrations provide researchers with confidence in achieving accurate, sensitive, and reproducible amino acid composition analysis using HPLC or amino acid analyzers.
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