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Specifications:
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Analytical grade | Formula | C₁₀H₁₆N₂O₈ |
Thermo Scientific™ Pierce EDTA (ethylenediaminetetraacetic acid) is a high-purity chelating agent used extensively in biochemistry, analytical chemistry, and molecular biology for the sequestration of divalent and trivalent metal ions, including calcium, magnesium, copper, zinc, and iron. With a purity of ≥99%, this versatile reagent is suitable for use in buffer preparation, enzyme assays, protein purification, and other laboratory protocols requiring precise control of metal ion concentrations.
Supplied in powder form and available in a 500 g unit, this EDTA salt is stable at room temperature and readily soluble in aqueous solutions (with pH adjustment), making it a staple reagent in both academic and industrial laboratories.
Key Features & Benefits
✅ ≥99% Purity – Research-grade quality for consistent results in sensitive experiments
✅ Chelates a Broad Range of Metals – Binds alkaline earth metals (Ca²⁺, Mg²⁺) and transition metals (Fe³⁺, Cu²⁺, Zn²⁺)
✅ Versatile Reagent – Commonly used in nucleic acid extraction, enzymatic stabilization, and titrimetric analysis
✅ Stable, Easy-to-Handle Powder – Long shelf life and flexible for custom concentration preparations
✅ Thermo Scientific Quality – Backed by rigorous quality control and documentation standards
Applications
- Buffer Preparation – Tris-EDTA (TE buffer), TBS, PBS formulations
- DNA and RNA Purification – Protects nucleic acids from metal-dependent nucleases
- Protein Purification – Chelates metal ions to prevent degradation and aggregation
- Enzyme Assays – Stabilizes sensitive enzymes by reducing metal-catalyzed oxidation
- Colorimetric and Redox Assays – Used in complexometric titrations and reaction control
- Cell Culture – Component in tissue dissociation and buffer solutions
Technical Specifications
| Attribute | Details |
|---|---|
| Product Name | EDTA, Disodium Salt, Dihydrate (Na₂EDTA·2H₂O) |
| Catalog Number | 17892 |
| Chemical Name | Ethylenediaminetetraacetic Acid, Disodium Salt, Dihydrate |
| Molecular Weight | 372.24 g/mol |
| CAS Number | 6381-92-6 |
| Purity | ≥99% |
| Form | White crystalline powder |
| Quantity | 500 g |
| Storage Conditions | Room temperature (cool, dry, ventilated area) |
| Shelf Life | Stable under recommended storage |
| Shipping Conditions | Ambient |
| Product Type | Chelating agent |
| Regulatory Use | For Research Use Only – Not for diagnostic use |
Storage & Handling
- Store in original container in a cool, dry, well-ventilated area away from moisture
- Seal tightly after use to prevent clumping or contamination
- For solution preparation, adjust pH to 8.0–9.0 using NaOH for optimal solubility
Thermo Scientific™ Pierce EDTA delivers trusted, high-quality chelation performance for a wide variety of research applications. Whether used in buffer systems, DNA extraction, protein stabilization, or enzyme preservation, this reagent offers consistent purity, reliable binding of metal ions, and flexible usability in critical laboratory workflows.