Gibco™ McCoy's 5A (Modified) Medium

The Gibco™ McCoy's 5A (Modified) Medium is a versatile cell culture medium designed to support the propagation of various primary cells, established cell lines, and explants derived from biopsy tissues. This medium provides the essential nutrients and buffering system required for robust cell growth, making it ideal for general-purpose applications in research and cell culture workflows.

Key Features:

1. Wide Compatibility:
   • Supports the growth of primary mammalian cells from tissues such as bone marrow, skin, spleen, kidney, lung, and rat embryos.
   • Effective for established cell lines and biopsy tissue explants.
2. Optimized Formulation:
   • Contains high glucose for enhanced energy metabolism.
   • Includes L-glutamine for protein synthesis and cell viability.
   • Incorporates phenol red as a pH indicator.
3. Serum Supplementation Required:
   • Designed for use with 10% Fetal Bovine Serum (FBS) or other serum supplements.
4. Sodium Bicarbonate Buffer System:
   • Requires a 5–10% CO₂ environment to maintain physiological pH levels.
5. Sterile and Stable:
   • Sterile-filtered for contamination-free cultures.
   • Long shelf life of 12 months when stored at 2–8°C and protected from light.

Modifications:

Applications:

1. Primary Cell Cultures:
   • Supports the propagation of cells derived from mammalian tissues such as skin, lung, spleen, and bone marrow.
2. Established Cell Lines:
   • Maintains the growth and health of rat fibroblasts and other cell lines.
3. Tissue Explants:
   • Facilitates the growth of biopsy tissues for diagnostic and research purposes.
4. General Research:
   • Suitable for experiments in cell biology, toxicology, and drug discovery.

Specifications:

Use Cases:

1. Energy-Intensive Cell Cultures:
   • High-glucose formulation supports cells with elevated metabolic demands.
2. Diagnostic Applications:
   • Maintains explants derived from biopsy tissues for short-term diagnostics.
3. Tissue-Specific Research:
   • Ideal for studies involving tissue-specific primary cells and cell lines.
4. Experimental Flexibility:
   • Compatible with various experimental setups, including CO₂-incubated and standard laboratory conditions.

Advantages:

1. Versatility:
   • Supports a broad range of cell types and applications.
2. Reliable Performance:
   • Manufactured under cGMP-compliant and ISO 13485-certified standards, ensuring high-quality and reproducible results.
3. Nutrient-Rich:
   • Enriched with essential nutrients like L-glutamine and high glucose to sustain robust cell growth.
4. Long Shelf Life:
   • Stable for up to 12 months under recommended storage conditions.

Storage and Handling:

• Storage: Keep at 2–8°C, protected from light to maintain nutrient stability.
• Shelf Life: 12 months from the date of manufacture.
• Usage: Thaw completely and mix thoroughly before use. Supplement with FBS or other serum as required.

Product Details:

The Gibco™ McCoy's 5A (Modified) Medium provides a reliable and nutrient-rich environment for cell culture applications, from primary cell propagation to tissue explant maintenance. Its optimized formulation and broad compatibility make it a go-to solution for researchers in cell biology and related fields.