Gibco™ HBSS (10X), calcium, magnesium, no phenol red

Gibco™ Hanks' Balanced Salt Solution (HBSS) (10X) with calcium and magnesium, without phenol red, is a concentrated, sterile-buffered salt solution designed for a variety of cell culture applications, including washing cells, tissue transport, reagent preparation, and dissociation.

The presence of calcium and magnesium supports cell adhesion and intercellular interactions, making this formulation ideal for maintaining cell integrity during washing and transport. Since it is a 10X concentrate, it requires dilution, pH adjustment, and filtration before use.

Key Features & Benefits

1. Concentrated 10X Formulation for Customization & Flexibility
   • Requires dilution before use, allowing customization for specific applications.
   • Ideal for laboratories preparing large volumes of working solution.
2. Contains Calcium & Magnesium for Cell Integrity
   • Supports cell-cell interactions and tissue stability.
   • Prevents excessive cell dissociation during processing.
3. Phenol Red-Free for Sensitive Applications
   • Reduces potential background interference in fluorescence assays & live-cell imaging.
   • Ideal for microscopy and immunohistochemistry (IHC) studies.
4. Sterile, Pre-Filtered, and cGMP-Compliant
   • Manufactured under ISO 13485 standards for high-quality research use.
   • Ensures consistent experimental results with long-term stability (24 months shelf life).
5. Supports Multiple Cell Culture Applications
   • Used for cell washing, tissue transport, dilution, and buffer preparation.
   • Essential for serum-free and custom cell culture media formulation.

Product Specifications

Use Cases & Applications

1. Cell Washing & Tissue Transport

• Prepares Cells for Downstream Experiments
  • Maintains osmotic balance and cell viability during processing.
  • Suitable for washing cells before media changes or cryopreservation.
• Ideal for Tissue Transport & Storage
  • Prevents osmotic shock and cell stress when transporting tissue samples.
  • Used in biopsies and primary cell research workflows.

2. Custom Cell Culture Media & Reagent Preparation

• Flexible Buffer for Custom Media Formulation
  • Supports serum-free and chemically defined cell culture conditions.
  • Used as a base for nutrient dilution and supplement preparation.
• Optimized for High-Throughput Laboratories
  • Suitable for automated liquid handling systems and media preparation.
  • Enhances reproducibility and efficiency in large-scale cell culture workflows.

3. Cell Isolation, Expansion, & Bioprocessing

• Maintains Cell Integrity in Adherent & Suspension Cultures
  • Provides essential ions for cell adhesion and intercellular interactions.
  • Reduces shear stress during washing and dissociation steps.
• Supports Stem Cell & Organoid Culture
  • Compatible with neural, cardiac, and epithelial stem cell research.
  • Used in co-culture systems and regenerative medicine applications.

4. Immunohistochemistry (IHC) & Functional Assays

• Supports Cell Fixation & Staining Protocols
  • Provides a stable, physiologically buffered environment for sample processing.
  • Reduces nonspecific cell lysis during antibody labeling procedures.
• Ensures Optimal Cell Functionality in Physiological Conditions
  • Maintains pH balance and electrolyte homeostasis.
  • Essential for preclinical drug screening and cytotoxicity assays.

Instructions for Use

Preparation of Working Solution (1X)

1. Dilution:
   • Dilute 1 part 10X HBSS to 9 parts sterile water to achieve a 1X working solution.
2. pH Adjustment:
   • Adjust pH to 7.2 – 7.4 using sodium bicarbonate or HEPES buffer.
3. Sterilization (if required):
   • Filter sterilize through a 0.22 µm membrane for use in sterile cell culture applications.
4. Storage:
   • Store prepared 1X HBSS at 4°C and use within 2–4 weeks for best performance.

Storage & Handling Instructions

• Storage Conditions:
  • Store 10X HBSS at 15–30°C.
  • Once diluted to 1X, store at 4°C and use within 2–4 weeks.
• Handling Precautions:
  • Use aseptic techniques to prevent contamination.
  • Ensure proper mixing before use to maintain uniform ion distribution.
• Disposal Guidelines:
  • Dispose of used media according to institutional biosafety protocols.
  • Autoclave if required before disposal.

Why Choose Gibco™ HBSS (10X), Calcium, Magnesium, No Phenol Red?

✅ Highly concentrated (10X) formulation for bulk preparation & flexibility

✅ Contains calcium & magnesium for optimal cell integrity & adhesion

✅ Sterile, pre-filtered, ready-to-use with cGMP compliance

✅ Phenol red-free to reduce assay interference & background noise

✅ Long shelf life (24 months) with stable storage at room temperature

Gibco™ HBSS (10X), Calcium, Magnesium, No Phenol Red is an essential solution for cell culture workflows, reagent preparation, and primary cell isolation, ensuring high reproducibility and minimal interference in molecular biology and biopharmaceutical applications.