Invitrogen™ eBioscience™ CD199 (CCR9) Monoclonal Antibody (BBC3M4), PE, 100 Tests

The Invitrogen™ CD199 (CCR9) Monoclonal Antibody (BBC3M4), conjugated to PE (Phycoerythrin), is a high-quality antibody designed for detecting CD199 (CCR9) on human cells. CCR9 is a G-protein-coupled receptor that plays a key role in thymocyte development and immune cell trafficking to gut-associated tissues. This antibody is optimized for flow cytometry applications, providing bright fluorescence for accurate identification of CCR9-expressing cells.

Key Features:

1. Target and Specificity:
   • Recognizes CD199 (CCR9), a receptor expressed on thymocytes and immune cells involved in gut-associated tissue trafficking.
   • High specificity ensures reliable detection in flow cytometric studies.
2. Clone and Conjugate:
   • Clone: BBC3M4, a trusted and validated clone for CCR9 detection.
   • Conjugate: PE (Phycoerythrin), offering bright fluorescence with excitation at 565 nm and emission at 576 nm.
3. Validated Applications:
   • Designed for flow cytometry, enabling robust analysis of CCR9 expression in human immune cells.
4. Bright and Stable Fluorescence:
   • PE provides high signal intensity, making it ideal for detecting low-abundance targets like CCR9.
5. Pre-Titrated and Ready-to-Use:
   • Requires only 5 µL (0.125 µg) per test, simplifying experimental workflows.

Specifications:

Applications:

1. Flow Cytometry:
   • Detect surface CCR9 expression in human thymocytes, CD4+ T cells, and other immune cell subsets.
   • Analyze immune cell trafficking to gut-associated lymphoid tissues (GALT).
2. Thymocyte Development Studies:
   • Investigate CCR9’s role in thymocyte migration and maturation during development.
3. Gut-Associated Immunity:
   • Study the upregulation of CCR9 in response to dendritic cell-derived retinoic acid and its involvement in gut immunity.
4. Chemokine Receptor Research:
   • Analyze CCR9's interaction with its ligand CCL25 (TECK) and its role in immune cell localization.

Key Benefits:

1. Bright and Stable Fluorescence:
   • PE ensures high signal intensity for clear separation of CCR9-positive and negative populations.
2. Reproducible Results:
   • Pre-titrated and validated for flow cytometry, providing consistent and reliable data.
3. Versatility:
   • Suitable for studying thymocyte development, gut immunity, and chemokine receptor pathways.
4. Ease of Use:
   • Ready-to-use liquid formulation simplifies experimental workflows.
5. High Specificity:
   • The BBC3M4 clone offers reliable detection of CCR9 in human immune cells.

Usage Recommendations:

1. Sample Preparation:
   • Isolate human CD4+ T cells or other CCR9-expressing cells from peripheral blood or tissue samples.
2. Staining Protocol:
   • Add 5 µL (0.125 µg) of antibody per 100 µL of cell suspension.
   • Incubate with cells in the dark for 20–30 minutes at 4°C or room temperature.
3. Flow Cytometry:
   • Analyze stained samples using a flow cytometer equipped with a blue laser (488 nm), green laser (561 nm), or yellow-green laser.
4. Controls:
   • Use an isotype control (e.g., Mouse IgG1 kappa Isotype Control, PE) for gating and specificity assessment.

Storage and Handling:

• Store at 4°C in the dark to protect the PE fluorochrome.
• Do not freeze, as freezing may degrade the antibody or fluorochrome.

Target Information:

• CD199 (CCR9):
  • A G-protein-coupled receptor involved in thymocyte development and immune cell migration.
  • Plays a role in the localization of immune cells to the gut through interactions with its ligand, CCL25 (TECK).
  • Regulated during thymic development and upregulated in response to retinoic acid signaling.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.