Invitrogen™ Dynabeads™ Untouched™ Human CD8 T Cells Kit

The Invitrogen™ Dynabeads™ Untouched™ Human CD8 T Cells Kit is a highly efficient negative isolation system for isolating pure and viable untouched CD8+ T cells from human PBMCs (peripheral blood mononuclear cells). The kit depletes non-CD8 cells, including CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes, leaving untouched CD8+ T cells for functional assays or downstream applications.

Key Features:

1. Untouched and Viable CD8+ T Cells:
   • Isolated cells remain bead- and antibody-free, preserving their natural state for functional assays.
2. High Purity and Viability:
   • Provides over 95% viability and up to 98% purity of CD8+ T cells.
3. Negative Isolation Technology:
   • Depletes unwanted cell types using antibody-labeled Dynabeads™, leaving CD8+ T cells untouched.
4. No Columns Required:
   • Simplifies workflows by eliminating the need for additional separation devices.
5. Easy to Use and Scalable:
   • Processes up to 1 × 10⁹ cells total, supporting large-scale experiments.

Specifications:

Applications:

1. Flow Cytometry:
   • Analyze CD8+ T cell subsets for markers of activation, memory, and cytotoxicity.
2. Functional Assays:
   • Study T cell proliferation, cytokine secretion, and effector functions.
3. Immunotherapy Research:
   • Explore the role of CD8+ T cells in tumor immunity and adoptive T cell transfer.
4. Translational Research:
   • Evaluate CD8+ T cell responses in infectious diseases, autoimmunity, and cancer.
5. Basic Immunology:
   • Investigate CD8+ T cell biology, including cytotoxic mechanisms and antigen-specific responses.

Advantages:

1. Preserved Functionality:
   • Untouched CD8+ T cells are free of Dynabeads™ and antibodies, ensuring natural cellular behavior.
2. Efficient and Reproducible:
   • Delivers highly consistent results with minimal sample loss.
3. Flexible and Scalable:
   • Suitable for both small-scale and large-scale experiments.
4. Simplified Workflow:
   • Magnetic bead-based isolation eliminates complex procedures, allowing for faster processing.

Kit Components:

• 10 mL Depletion Dynabeads™: Targets and removes non-CD8 cells.
• 2 mL Antibody Mix: Contains monoclonal antibodies against CD4, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123, and CD235a (Glycophorin A).

Isolation Procedure:

1. Label Non-CD8 Cells:
   • Add the antibody mix to PBMCs and incubate to bind non-CD8 cells.
2. Bind Dynabeads™:
   • Add Dynabeads™ to the sample and incubate for a short period to bind antibody-labeled cells.
3. Magnetic Separation:
   • Place the sample on a magnetic separator. Bead-bound cells are retained, and untouched CD8+ T cells remain in the supernatant.
4. Collect CD8+ T Cells:
   • Transfer the CD8+ T cell-enriched supernatant for immediate use in downstream applications.

Ordering Information:

Storage and Handling:

• Storage: Store at 2°C–8°C. Avoid freezing.
• Shelf Life: Refer to product labeling for expiration date.

Related Products:

• DynaMag™ Magnets: For magnetic separation workflows.
• Dynabeads™ Magnetic Beads: Compatible with various isolation applications.
• Dynabeads™ Human CD8+ T Cell Positive Isolation Kit: For direct isolation of CD8+ T cells.

The Invitrogen™ Dynabeads™ Untouched™ Human CD8 T Cells Kit is a reliable and efficient solution for isolating pure, viable CD8+ T cells. Its negative isolation technology ensures bead-free and antibody-free cells, preserving their functionality for a wide range of research applications.