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Invitrogen™ TrackIt™ 100 bp DNA Ladder, 50 µg with 6X Loading Buffer
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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | -20°C | ||
| Product Type | DNA Ladders | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ TrackIt™ 100 bp DNA Ladder is a ready-to-use molecular weight marker designed for the accurate sizing and approximate quantification of double-stranded DNA fragments ranging from 100 to 2,000 base pairs. This ladder consists of 13 chromatography-purified DNA fragments, with reference bands at 2,000 bp, 1,500 bp, and 600 bp to facilitate easy identification and orientation during electrophoresis.
Each band contains a defined amount of DNA, enabling both visual sizing and approximate quantification when run alongside experimental samples. The ladder is pre-mixed with 6X TrackIt™ Cyan/Orange Loading Buffer, providing enhanced visibility and tracking during agarose gel electrophoresis.
Key Features and Benefits
- 13 Sharp, Distinct Bands: Clean, consistent bands from 100 bp to 2,000 bp
- Reference Bands: Enhanced visual orientation at 600 bp, 1,500 bp, and 2,000 bp
- Ready-to-Use Format: Pre-diluted and pre-mixed with 6X TrackIt Cyan/Orange Loading Buffer
- Quantifiable Bands: Known mass of DNA per band for estimating sample concentration
- Stable at Room Temperature: 24-month shelf life with no need for immediate refrigeration
- Compatible with Common Stains: Visualize with ethidium bromide or SYBR Safe
- Ideal for Agarose Gels: Recommended for use with 1–2% agarose gels
Applications
- DNA fragment size estimation in PCR, cloning, and restriction digestion workflows
- Routine agarose gel electrophoresis
- Approximate quantification of dsDNA samples
- Verification of amplicons or plasmid digests
- Educational demonstrations and teaching labs
Technical Specifications
| Attribute | Details |
|---|---|
| Product Type | DNA Ladder |
| DNA Type | Double-stranded DNA |
| Size Range | 100 bp to 2,000 bp |
| Number of Bands | 13 |
| Concentration | 0.1 µg/µL |
| Volume Provided | 500 µL |
| Loading Dye | 6X TrackIt Cyan/Orange (1 mL included) |
| Gel Compatibility | 1–2% agarose gels |
| Visualization Methods | Ethidium bromide, SYBR Safe |
| Storage Conditions | Room temperature or 4°C (up to 24 months) |
| Shipping Conditions | Room temperature or dry ice |
| Green Features | Sustainable packaging |
| Product Line | TrackIt™ |
Kit Contents
- 500 µL TrackIt™ 100 bp DNA Ladder
- 1 mL 6X TrackIt™ Cyan/Orange Loading Buffer
The Invitrogen™ TrackIt™ 100 bp DNA Ladder is a convenient and reliable tool for DNA fragment analysis, offering high-resolution band clarity, built-in reference bands, and room temperature stability. It supports accurate sizing and quantification in routine molecular biology workflows and educational settings.