Gibco™ Penicillin-Streptomycin-Glutamine (100X)

The antibiotic solution containing penicillin, streptomycin, and L-glutamine is an essential reagent for preventing bacterial contamination in cell cultures. It is specifically formulated to target gram-positive and gram-negative bacteria, combining effective antibacterial activity with enhanced cell culture support through L-glutamine supplementation.

Composition

• Penicillin: 10,000 units/mL
  • Sourced from Penicillium fungi.
  • Interferes with bacterial cell wall synthesis, leading to structural instability and bacterial death.
  • Indirectly triggers enzymes that further degrade bacterial cell walls.
• Streptomycin: 10,000 μg/mL
  • Derived from Streptomyces griseus.
  • Binds to the 30S ribosomal subunit, inhibiting protein synthesis and causing bacterial death.
• L-Glutamine: 29.2 mg/mL
  • Provides essential nutrients for cell metabolism.
  • Stabilized with 10 mM citrate buffer to maintain pH consistency.

Features and Benefits

1. Broad-Spectrum Antibacterial Action:
   • Targets both gram-positive and gram-negative bacteria, effectively preventing contamination in mammalian and insect cell cultures.
2. Nutritional Support:
   • L-glutamine ensures optimal cell growth and viability by serving as a crucial energy source.
3. High Purity and Stability:
   • The solution is sterile-filtered to ensure contamination-free usage.
   • Buffered for enhanced pH stability, preventing degradation during storage and usage.
4. Convenience:
   • Supplied as a 100X concentrate, reducing the need for frequent preparation.
   • Pre-mixed combination reduces handling steps, saving time and effort.
5. Long Shelf Life:
   • Stable for up to 12 months when stored between -5°C to -20°C.
   • Ships on dry ice to preserve integrity during transit.

Specifications

Applications

• Contamination Control:
  • Prevents bacterial contamination in mammalian and insect cell cultures.
• Cell Selection:
  • Suitable for eukaryotic and bacterial selection in co-culture systems.
• Research Use:
  • Ideal for preclinical research and laboratory workflows.

Usage Instructions

1. Dilution: Add the antibiotic solution to the cell culture medium to achieve a 1X final working concentration:
   • Penicillin: 100 U/mL
   • Streptomycin: 100 μg/mL
   • L-Glutamine: 0.292 mg/mL
2. Mixing: Ensure thorough mixing of the solution in the medium before use.
3. Storage: After dilution, the medium can be stored at 2-8°C for short-term use or used immediately.

Key Applications

• Mammalian Cell Culture: Effective in contamination prevention for hybridomas, HEK293, CHO, and other common cell lines.
• Insect Cell Culture: Supports Sf9 and Sf21 cell growth without contamination.
• Tissue Engineering: Maintains sterility in scaffold-based cultures.

For Research Use Only

• This product is strictly for laboratory applications and not intended for diagnostic use.

By combining broad-spectrum antibacterial activity with L-glutamine, this solution addresses both contamination control and cell culture optimization, making it an indispensable tool in cell culture workflows.