Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Optimized Reagent System for Intracellular Detection of Transcription Factors and Nuclear Proteins

The Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set is a complete, validated solution for intracellular staining of transcription factors and nuclear antigens such as Foxp3, Ki-67, and cytokines. This buffer system is specifically optimized for use with fluorochrome-conjugated antibodies in flow cytometric analysis, enabling accurate detection of low-abundance or intracellularly localized targets.

The kit includes all necessary buffers for fixation and permeabilization and is particularly effective for identifying regulatory T cells (Tregs) through Foxp3 expression.

Key Features and Benefits

• Complete Buffer Set for Intracellular Staining
  Includes fixation and permeabilization reagents and diluents, eliminating the need for separate components.
• Optimized for Transcription Factors
  Designed for sensitive detection of nuclear and intranuclear proteins such as Foxp3 and Ki-67.
• Compatible with Multicolor Flow Cytometry
  Maintains fluorochrome integrity, including tandem dyes, for use in high-dimensional immune profiling.
• Validated in Treg and Proliferation Studies
  Trusted for accurate detection of regulatory T cell markers, cell proliferation, and immune activation.

Applications

• Intracellular staining of transcription factors (e.g., Foxp3, Ki-67)
• Flow cytometric analysis of regulatory T cells (Tregs)
• Proliferation assays and cytokine expression analysis
• Immune cell subset characterization and functional studies

Technical Specifications

Kit Components

• Fixation/Permeabilization Concentrate
• Fixation/Permeabilization Diluent
• Permeabilization Buffer (10X)

All components have been optimized for use together. For individual reagents, refer to:

• Fixation/Permeabilization Conc. + Diluent: Cat. No. 00-5521-00
• Permeabilization Buffer (10X): Cat. No. 00-8333-56

The Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set is the gold standard for intracellular flow cytometry workflows, especially in Treg biology and immune regulation research. It provides a reliable, consistent protocol for staining nuclear targets while preserving fluorochrome brightness and cell viability.