Sigma-Aldrich Vancomycin hydrochloride from Streptomyces orientalis ≥85% (Vancomycin B)

Validated for Microbiology, Antimicrobial Resistance Research, and Drug Development

Sigma-Aldrich Vancomycin Hydrochloride (≥85%, Vancomycin B) is a high-purity glycopeptide antibiotic derived from Streptomyces orientalis. It is a powerful inhibitor of bacterial cell wall synthesis, making it effective against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile.

This high-potency, endotoxin-tested antibiotic is widely used in microbiology, pharmaceutical research, and antimicrobial resistance studies. Its broad-spectrum activity makes it essential for evaluating antibiotic efficacy, bacterial susceptibility, and clinical microbiology applications.

Key Features & Benefits

✅ High-Purity Glycopeptide Antibiotic (≥85% Vancomycin B) – Ensures consistent and reproducible antimicrobial activity

✅ Broad-Spectrum Activity Against Gram-Positive Bacteria – Effective against Streptococcus, Staphylococcus, and Enterococcus species

✅ Validated for Antimicrobial Resistance Research – Used in drug development, MIC testing, and bacterial susceptibility studies

✅ Inhibits Bacterial Cell Wall Synthesis – Prevents D-alanyl-D-alanine incorporation into peptidoglycan chains

✅ Highly Soluble in Water (50 mg/mL) – Ensures easy preparation of antibiotic stock solutions

✅ Stable Under Proper Storage Conditions (2–8°C) – Maintains long-term potency and efficacy

✅ Available in Multiple Pack Sizes – Suitable for small-scale laboratory testing and large-scale research applications

Technical Specifications

Mechanism of Action & Resistance

Mode of Action:

• Vancomycin inhibits bacterial cell wall synthesis by binding to D-alanyl-D-alanine terminal residues in peptidoglycan precursors, preventing cross-linking and cell wall formation.
• This results in osmotic instability and bacterial lysis, making it highly effective against Gram-positive bacterial infections.

Mode of Resistance:

• Vancomycin-resistant bacteria (VRE - Vancomycin-resistant Enterococcus) modify the D-alanyl-D-alanine binding site, reducing drug affinity.
• Some bacteria produce thicker peptidoglycan layers, limiting Vancomycin access to its target sites.

Antimicrobial Spectrum:

✔ Gram-Positive Bacteria (Staphylococcus aureus, Streptococcus pneumoniae, Clostridium difficile, Enterococcus spp.)

✔ Methicillin-Resistant Staphylococcus aureus (MRSA)

✔ Vancomycin-Sensitive Enterococcus faecalis and Enterococcus faecium

Applications

🔬 Microbiology & Bacterial Culture Research – Used for bacterial growth inhibition studies

🦠 Antimicrobial Susceptibility Testing (AST) – Determines bacterial resistance to Vancomycin

⚗️ Pharmaceutical Drug Development – Supports research on glycopeptide antibiotics & bacterial resistance

🧪 Quality Control & Biopharmaceutical Research – Used in antibiotic formulation and validation studies

🏥 Clinical Microbiology & Diagnostic Testing – Evaluates Vancomycin resistance in MRSA & enterococci

Preparation & Storage Guidelines

1️⃣ Preparation of Stock Solution

• Dissolve 50 mg/mL in sterile water or PBS.
• Filter sterilization recommended (do not autoclave).
• Store at 2–8°C for short-term use or -20°C for extended stability.

2️⃣ Recommended Working Concentrations

• Bacterial Inhibition in Microbiology Studies: 10–100 µg/mL
• Antimicrobial Susceptibility Testing (AST): Variable (refer to CLSI/EUCAST guidelines)

3️⃣ Storage Conditions

• Keep tightly sealed in a dry, ventilated environment.
• Protect from moisture & high temperatures to prevent degradation.
• Avoid repeated freeze-thaw cycles for optimal stability.

Ordering Information

Regulatory & Safety Information

⚠️ For Research Use Only (RUO). Not intended for clinical or therapeutic applications.

⚠️ Hygroscopic & Light-Sensitive – Store in a dry, well-ventilated area.

⚠️ Use Protective Equipment (PPE) – Avoid direct skin contact & inhalation.

Why Choose Sigma-Aldrich Vancomycin Hydrochloride (≥85%, Vancomycin B)?

🔹 High-Purity Glycopeptide Antibiotic (≥85%) for Reliable Research

🔹 Effective Against Gram-Positive Bacteria, Including MRSA & Streptococcus

🔹 Validated for Pharmaceutical Research, Antimicrobial Studies, and Clinical Microbiology

🔹 Stable Under Proper Storage Conditions (2–8°C)

🔹 Available in Multiple Pack Sizes for Research Flexibility

For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀