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Invitrogen™ SYTOX™ Dead Cell Stain Sampler Kit, for flow cytometry, 5 x 50 μL
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50 µL in DMSO vial each of:
SYTOX™ Blue
SYTOX™ Green
SYTOX™ Orange
SYTOX™ Red
SYTOX™ AADvanced™
Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | -20°C | ||
| Product Type | Flow Cytometry Reagents | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Multiplex-Compatible Dead Cell Stains for Flow Cytometry – 5 Colors, One Kit
The SYTOX™ Dead Cell Stain Sampler Kit includes five validated, cell-impermeant nucleic acid stains optimized for flow cytometric discrimination of live and dead cells. These stains bind nucleic acids in membrane-compromised (dead) cells, emitting bright fluorescence with minimal background—ideal for no-wash, single-step viability assays.
Designed for use across multiple laser lines (violet, blue, green, and red), the sampler kit includes SYTOX™ Blue, Green, Orange, AADvanced™, and Red, providing flexibility for panel design and viability gating in multicolor experiments.
Key Features and Benefits
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Includes 5 Spectrally Distinct Dyes
Easily identify the optimal viability dye for your multicolor flow cytometry panel:- SYTOX™ Blue – 405 nm excitation / 480 nm emission
- SYTOX™ Green – 488 nm excitation / 523 nm emission
- SYTOX™ Orange – 532 nm excitation / 570 nm emission
- SYTOX™ AADvanced™ – 488 nm excitation / 647 nm emission
- SYTOX™ Red – 633 nm excitation / 658 nm emission
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Single-Step, No-Wash Assay
Simply add dye, incubate briefly, and analyze—no wash or fixation required. -
Validated Across Cell Types and Applications
Proven in Jurkat, HeLa, HEK, CHO, 3T3, ReH, Kg1a, and lysed whole blood; suitable for apoptosis, proliferation, immunophenotyping, and cell cycle assays. -
Bright Signal with Low Background
Non-fluorescent in aqueous media; minimal background improves gating precision. -
Flexible Trial Format
Small-volume (50 µL each) vials allow side-by-side testing of multiple dyes before scaling up.
Applications
- Live/dead cell discrimination in flow cytometry
- Cell viability analysis in multiparameter panels
- Apoptosis detection and cytotoxicity studies
- Immunophenotyping of fresh or cultured samples
- Cell cycle and proliferation assays
Technical Specifications
| Property | Details |
|---|---|
| Product Name | SYTOX™ Dead Cell Stain Sampler Kit |
| Catalog Number | S34862 |
| Form | Solution (DMSO) |
| Quantity | 5 × 50 µL vials (1 vial per dye) |
| Dyes Included | SYTOX™ Blue, Green, Orange, AADvanced™, Red |
| Excitation (Range) | 350–640 nm (varies by dye) |
| Emission (Range) | 480–658 nm |
| Detection Method | Fluorescence |
| Cell Permeability | Impermeant (labels dead cells only) |
| Subcellular Target | Nucleus, nucleic acids |
| Instrument Compatibility | Flow cytometer with violet, blue, green, or red lasers |
| Storage Conditions | ≤ –20°C, desiccated, protect from light |
| Shipping Conditions | Room temperature |
| Product Line | SYTOX™, Molecular Probes™ |
| Unit Size | Each (1 kit) |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Kit Contents
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1 vial each of:
- SYTOX™ Blue
- SYTOX™ Green
- SYTOX™ Orange
- SYTOX™ Red
- SYTOX™ AADvanced™
- Total: 5 × 50 µL in DMSO
The SYTOX™ Dead Cell Stain Sampler Kit offers a flexible, cost-effective way to evaluate five bright, reliable dead cell stains across different laser lines. Perfect for researchers optimizing high-parameter flow cytometry panels, this kit ensures accurate live/dead gating with minimal spectral overlap and simple, no-wash protocols.