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Applied Biosystems™ RNase Inhibitor, 2000 U, 20 U/μL, 100 Rxns
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Specifications:
| Application | PCR | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
The Applied Biosystems™ RNase Inhibitor is a high-quality recombinant ribonuclease inhibitor enzyme designed to protect RNA molecules from degradation during RNA-based molecular biology workflows. With a molecular weight of approximately 50 kDa, this enzyme specifically inhibits RNase activity without affecting DNA or enzymatic reactions involving DNA, ensuring optimal preservation of RNA integrity during sensitive applications such as reverse transcription, RNA sequencing, and RT-PCR.
RNA is highly susceptible to degradation by ubiquitous RNases present in laboratory environments, reagents, and biological samples. This RNase inhibitor binds tightly to RNases and effectively blocks their activity, preventing RNA template degradation and ensuring accurate downstream analysis. Its lack of DNase or endonuclease activity makes it especially suitable for applications requiring high RNA purity and integrity.
Supplied at a concentration of 20 U/μL, the enzyme provides reliable performance across multiple reactions and is sufficient for approximately 100 reverse transcription reactions, making it an essential reagent in molecular biology, genomics, transcriptomics, and RNA sequencing workflows.
Key Features
- Effective RNase inhibition: Protects RNA from degradation by RNases
- Recombinant enzyme: High purity and consistent performance
- DNase-free and endonuclease-free: Ensures preservation of DNA integrity and accurate results
- Essential for RNA workflows: Supports reverse transcription, RNA sequencing, and RT-PCR applications
- High activity concentration: 20 U/μL enzyme concentration for efficient RNA protection
- Improves experimental reliability: Ensures accurate RNA analysis and reproducible results
- Compatible with multiple workflows: Suitable for manual and automated applications
Applications
- Reverse transcription (RT) reactions
- RT-PCR and qRT-PCR workflows
- RNA sequencing (RNA-Seq) workflows
- cDNA synthesis
- RNA library preparation
- Transcriptomics research
- RNA isolation and handling
- Molecular biology and genomics research
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | RNase Inhibitor |
| Enzyme Type | Recombinant ribonuclease inhibitor |
| Molecular Weight | 50 kDa |
| Concentration | 20 U/μL |
| Total Activity | 2,000 units |
| Reaction Volume | Supports ~100 RT reactions |
| Storage Buffer | 20 mM HEPES-KOH (pH 7.6), 50 mM KCl, 8 mM DTT, 50% glycerol |
| DNase Activity | None |
| Endonuclease Activity | None |
| Shipping Condition | Dry Ice |
| Storage Condition | Store at –20°C |
| Unit Size | 100 μL |
Workflow Integration
RNA Extraction → RNA Quantification → RNase Inhibition → Reverse Transcription → cDNA Synthesis → Library Preparation → Sequencing
Advantages
- Prevents RNA degradation and preserves sample integrity
- Improves reliability and accuracy of RNA-based experiments
- Enhances efficiency of reverse transcription and RNA sequencing workflows
- Ensures high-quality cDNA synthesis
- Essential for sensitive transcriptomic and gene expression analysis
The Applied Biosystems™ RNase Inhibitor is a critical reagent for protecting RNA integrity during molecular biology and RNA sequencing workflows. Its high specificity, reliable RNase inhibition, and DNase-free formulation make it an essential component for reverse transcription, RNA library preparation, and transcriptomics research, ensuring accurate, reproducible, and high-quality experimental results.