Applied Biosystems™ RNase Inhibitor, 2000 U, 20 U/μL, 100 Rxns

The Applied Biosystems™ RNase Inhibitor is a recombinant 50 kDa protein that provides robust protection against RNase activity in RNA workflows. It binds specifically to RNase A-type enzymes, inhibiting their catalytic activity and preserving RNA integrity during critical applications such as reverse transcription, RT-PCR, cDNA synthesis, and RNA storage.

Formulated in a stabilizing buffer with HEPES-KOH, KCl, DTT, and 50% glycerol, the inhibitor is free of DNase and endonuclease activity, making it a reliable addition to sensitive RNA research workflows.

Key Features

✅ Powerful RNase Inhibition

• Protects RNA from degradation by RNase A, RNase B, and RNase C
• Preserves template quality in reverse transcription and RT-PCR

✅ High Purity & Specificity

• No DNase or endonuclease activity, ensuring pure cDNA synthesis and high yields

✅ Optimized for Molecular Biology Applications

• Ideal for use with reverse transcriptases, RNA preps, RNA-seq library prep, and in vitro transcription reactions

✅ Ready-to-Use & Convenient Format

• Supplied at 20 U/μL
• Easy to use: Add 1 µL per 20 µL reverse transcription reaction

✅ Stabilized Storage Buffer

• Long shelf life at –20°C
• Formulated for consistency across experiments

Specifications

Applications

🧬 Reverse Transcription (RT)

• Use with reverse transcriptase to prevent RNA degradation during cDNA synthesis

🔬 RT-PCR / qRT-PCR

• Ensures accurate and consistent quantification of gene expression

📦 RNA Storage & Handling

• Add to RNA samples during storage or purification to protect from ambient RNase contamination

🔧 In Vitro Transcription & RNA Library Prep

• Maintains RNA integrity during long workflows

Contents & Storage

• 100 µL RNase Inhibitor (20 U/µL)
• Storage buffer:
  • 20 mM HEPES-KOH (pH 7.6)
  • 50 mM KCl
  • 8 mM DTT
  • 50% (v/v) glycerol
• Store at –20°C
• Sufficient for 100 reverse transcription reactions (20 µL reaction size)