Liofilchem® TTC 1% Supplement

Liofilchem® TTC 1% Supplement is a liquid microbiological reagent containing an aqueous solution of Triphenyltetrazolium chloride (TTC). It serves as a redox indicator in selective and differential culture media to visually distinguish microbial growth. The reagent is colorless in its oxidized form and converts into red insoluble triphenylformazan upon reduction by many bacteria and fungi, providing a clear and persistent color change.

Key Features:

1. Redox Indicator: TTC is reduced to triphenylformazan, indicating active microbial growth.
2. Irreversible Reaction: Once reduced, the red color persists, ensuring stable results.
3. Wide Application:
   • Compatible with various microbiological media including:
     • Slanetz and Bartley Agar Base (ref. 610134/620134).
     • Azide Maltose Agar KF (ref. 610154/620154).
     • Barnes Agar Base (ref. 610156).
     • Tergitol Agar Base (ref. 610184/620184).
4. Broad Microbial Use: Effective for bacteria and fungi differentiation in clinical, environmental, and food microbiology.
5. Pre-measured Convenience: Supplied in 10 mL tubes for easy and accurate preparation.

Applications:

1. Clinical Microbiology:
   • Identification and differentiation of bacteria and fungi in diagnostic workflows.
2. Environmental Monitoring:
   • Detection of microbial contamination in water and environmental samples.
3. Food and Beverage Testing:
   • Enumeration and identification of enterococci and other organisms in food quality control.
4. Industrial Microbiology:
   • Used in microbial monitoring in pharmaceutical and industrial environments.

Composition:

Preparation & Use:

1. Preparation:
   • Refer to the technical sheet of the base medium being used (e.g., Slanetz and Bartley Agar Base, Azide Maltose Agar KF, etc.).
   • Use one tube of TTC 1% Supplement (10 mL) per 1 liter of the medium.
2. Procedure:
   • Mix thoroughly after adding the supplement to the base medium.
   • Pour the prepared medium into Petri dishes or tubes as required.

Quality Control:

1. Visual Inspection:
   • The solution should be clear and colorless.
2. Microbiological Testing:
   • Prepare plates with Slanetz and Bartley Agar Base supplemented with TTC 1%.
   • Incubate plates at 36 ± 1°C for 48 hours.
   • Results:
     • Enterococcus faecalis (ATCC 29212): Good growth; red colonies.
     • Escherichia coli (ATCC 25922): Growth inhibited.

Performance Characteristics:

• Highly Specific: Detects microbial activity based on TTC reduction.
• Stable Results: Persistent red color ensures clear visualization of microbial activity.
• Compatible: Works with a variety of selective and differential media formulations.

Storage Conditions:

• Store at 2-8°C in the original packaging.
• Valid until the expiry date on the label, provided there are no signs of deterioration.

Precautions:

1. For professional use only.
2. Operators must be trained in microbiological procedures.
3. Read the Safety Data Sheet (SDS) for proper handling and safety information.
4. Dispose of waste according to local and national regulations.

Ordering Information:

Bibliography:

1. Kennor, G.A., Clark, H.F., and Kabler, P.W. (1961). J. Appl. Microbiol., 9: 15-20.
2. Slanetz, L.W., and Bartley, C.H. (1957). J. Bacteriol., 74: 591-595.
3. Chapman, G.H. (1947). J. Bacteriol., 53: 504.

Key Benefits:

• Simple and effective microbial detection.
• Versatile usage across multiple media types.
• Reliable redox reaction for microbial activity visualization.

Liofilchem® TTC 1% Supplement is a highly reliable tool for microbiologists, enabling precise microbial detection and differentiation in various fields, including clinical, food safety, and environmental testing.