Liofilchem® Cefotaxime CTX Antimicrobial Susceptibility Discs, 30 µg (copy)

Liofilchem® Amoxicillin-Clavulanic Acid (AUG) Antimicrobial Susceptibility Discs are precision-manufactured discs designed for susceptibility testing of microorganisms against the combination of amoxicillin and clavulanic acid. These discs are essential for identifying the effectiveness of this beta-lactam and beta-lactamase inhibitor combination, especially in detecting beta-lactamase-mediated resistance in both Gram-positive and Gram-negative bacteria. They are available in three distinct concentrations: 3 µg, 7.5 µg, and 30 µg.

Features and Composition:

1. Active Ingredients:
   • Amoxicillin: A broad-spectrum beta-lactam antibiotic effective against a wide range of Gram-positive and Gram-negative bacteria.
   • Clavulanic Acid: A beta-lactamase inhibitor that enhances the efficacy of amoxicillin against beta-lactamase-producing bacteria.
2. Disc Specifications:
   • Uniform and precise drug impregnation at 3 µg, 7.5 µg, and 30 µg.
   • High-quality filter paper ensuring consistent diffusion in agar-based media.
3. Quality Standards:
   • Manufactured under strict ISO and CLSI standards.
   • Compatible with CLSI and EUCAST guidelines for antimicrobial susceptibility testing.
4. Packaging:
   • Each cartridge contains 50 discs.
   • Discs are sealed with a desiccant to maintain stability and activity.
5. Shelf Life:
   • Long shelf life when stored at 2-8°C.
   • Stable under recommended storage conditions with desiccant protection.

Applications:

1. Clinical Use:
   • Testing beta-lactam and beta-lactamase inhibitor susceptibility in pathogenic bacteria.
   • Monitoring multidrug resistance in hospital and community-acquired infections.
2. Research:
   • Studying the efficacy of amoxicillin-clavulanic acid against beta-lactamase-producing organisms.
   • Investigating the synergy between amoxicillin and clavulanic acid in combating resistant strains.
3. Quality Control:
   • Standardized testing to ensure accurate results in antimicrobial susceptibility assays.
   • Suitable for use in laboratories following CLSI and EUCAST breakpoints.

Testing Procedure:

1. Disc Diffusion Method:
   • Prepare Mueller-Hinton agar plates according to CLSI/EUCAST standards.
   • Inoculate plates with a standardized bacterial suspension (0.5 McFarland turbidity).
   • Place the AUG discs on the agar surface using a sterile applicator.
   • Incubate plates at 35 ± 2°C for 16-20 hours.
2. Interpreting Results:
   • Measure the diameter of the zone of inhibition using a calibrated ruler.
   • Compare results to CLSI/EUCAST breakpoints to determine susceptibility.

Quality Control Strains:

• Escherichia coli ATCC® 25922 (Beta-lactamase negative control)
• Klebsiella pneumoniae ATCC® 700603 (ESBL-producing control)
• Staphylococcus aureus ATCC® 25923 (Gram-positive control)

Advantages:

1. Enhanced Sensitivity:
   • Clavulanic acid prevents beta-lactamase degradation of amoxicillin, ensuring activity against resistant strains.
2. Wide-spectrum Application:
   • Effective against beta-lactamase-producing strains, including Enterobacteriaceae, Pseudomonas aeruginosa, and Staphylococcus spp.
3. Precision and Reliability:
   • Uniform drug distribution ensures consistent results across tests.
4. Convenience:
   • Compatible with automated disc dispensers and manual application.

Storage and Stability:

• Store at 2-8°C in a sealed container with desiccant.
• Avoid exposure to moisture or high temperatures to maintain disc integrity.

Regulatory Compliance:

• For In Vitro Diagnostic Use (IVD):
  • Complies with ISO, CLSI, and EUCAST standards for antimicrobial susceptibility testing.
  • Approved for laboratory professional use.

Key Applications:

1. Detection of beta-lactamase-producing organisms.
2. Monitoring resistance in Gram-positive and Gram-negative pathogens.
3. Establishing beta-lactamase inhibitor efficacy in clinical and research settings.

Liofilchem® Amoxicillin-Clavulanic Acid AUG Antimicrobial Susceptibility Discs are essential tools for evaluating bacterial resistance mechanisms and optimizing antimicrobial therapy. With precise concentrations and compatibility with global standards, these discs provide reliable and reproducible results for clinical diagnostics and research applications.