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Liofilchem® Mueller Hinton Fastidious Broth 3.6 mL
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Liofilchem | ||
| Product Grade | Microbiology grade | ||
Liofilchem® Mueller Hinton Fastidious (MH-F) Broth is a ready-to-use, enriched liquid medium designed specifically for quantitative antimicrobial susceptibility testing (AST) by broth microdilution of fastidious organisms. The formulation is in accordance with EUCAST recommendations and supports the growth of nutritionally demanding bacteria, including Streptococcus pneumoniae, Haemophilus influenzae, Listeria monocytogenes, Campylobacter spp., and others.
This medium is essential in clinical microbiology labs for determining the minimum inhibitory concentration (MIC) of antibiotics against difficult-to-grow organisms, offering reliable and reproducible results in critical infectious disease diagnostics.
Key Features
- EUCAST-recommended formulation: Specifically designed for broth microdilution testing of fastidious organisms.
- Enriched composition: Based on cation-adjusted Mueller Hinton Broth with 5% lysed horse blood and 20 mg/L β-NAD.
- Optimized pH: Final pH of 7.3 ± 0.1 supports consistent microbial growth and accurate MIC determination.
- Ready-to-use packaging: Supplied in pre-aliquoted tubes (3.6 mL or 11 mL), minimizing preparation time and contamination risk.
- Validated with QC strains: Complies with ISO 20776-2:2021 and EUCAST internal QC protocols.
Typical Formula (per liter)
| Ingredient | Concentration |
|---|---|
| Beef Extract | 3.0 g |
| Acid Hydrolysate of Casein | 17.5 g |
| Starch | 1.5 g |
| β-NAD | 0.02 g (20 mg) |
| Lysed Horse Blood | 50.0 mL |
| Final pH at 25 °C | 7.3 ± 0.1 |
Applications and Use Cases
1. Clinical Microbiology
- MIC determination of fastidious pathogens such as S. pneumoniae, H. influenzae, L. monocytogenes, Moraxella catarrhalis, and others.
- Supports critical antibiotic susceptibility profiling for therapeutic guidance.
2. Antimicrobial Resistance Monitoring
- Used in surveillance of resistance patterns and validation of new antimicrobial compounds.
3. Pharmaceutical Testing
- MIC determination during new antimicrobial drug development and batch release quality control.
Test Procedure
- Antibiotic Dilution: Prepare two-fold serial dilutions of antimicrobial agents in MH-F broth.
- Inoculum Preparation: Adjust test organism suspension to standard turbidity (e.g., 0.5 McFarland).
- Inoculation: Add standardized inoculum to each dilution well or tube.
- Incubation: 35 ± 1°C for 16–20 hours under aerobic conditions.
- Interpretation: The MIC is the lowest concentration with no visible growth (turbidity). Interpret using EUCAST breakpoints.
Storage and Shelf Life
| Storage Conditions | Shelf Life |
|---|---|
| 2–8 °C, protected from light | 4 months from production |
Packaging Options
| Reference | Description | Format |
|---|---|---|
| 27507 | Mueller Hinton Fastidious Broth Tubes | 20 × 3.6 mL tubes |
| 21105 | Mueller Hinton Fastidious Broth Tubes | 20 × 11 mL tubes |
Quality Control
| Organism | Growth (≤100 CFU) | Incubation |
|---|---|---|
| Streptococcus pneumoniae ATCC® 49619 | Good growth | 16–20 h at 35 ± 1 °C |
| Haemophilus influenzae ATCC® 49766 | Good growth | 16–20 h at 35 ± 1 °C |
- Appearance: Ruby red, clear, no precipitate
Precautions & Limitations
- For in vitro diagnostic use only.
- Not intended for direct inoculation of clinical specimens.
- Always include a growth control for test validity.
- Interpretation must be done by trained professionals using current EUCAST/CLSI standards.
- The MIC in vitro does not always predict in vivo efficacy.
Liofilchem® Mueller Hinton Fastidious Broth is a high-performance, EUCAST-compliant liquid medium tailored for precise and reproducible MIC testing of fastidious organisms. Its standardized formulation ensures robust bacterial growth while supporting the needs of antimicrobial stewardship, clinical decision-making, and pharmaceutical testing environments.