Invitrogen™ BrightComp eBeads™ Compensation Bead Kit, 25 Tests

Optimized Compensation Beads for Fluorescent Protein-Expressing Samples in Flow Cytometry

BrightComp eBeads™ Compensation Bead Kits are specialized reagents designed for accurate flow cytometry compensation when working with fluorescent protein-expressing cells, such as those expressing GFP, RFP, YFP, CFP, or mCherry. Each kit contains polystyrene microspheres coated with spectrally matched fluorescent dyes that closely mimic the emission characteristics of native fluorescent proteins.

These kits provide a simple, consistent, and high-fidelity alternative to using cell-based controls, especially for weakly expressing or variable protein-expressing populations. Each drop includes three intensity levels and negative beads, enabling precise compensation setup across varying expression levels.

Key Features and Benefits

• Spectrally Matched to Native Fluorescent Proteins
  Ensures accurate compensation for GFP, RFP, YFP, CFP, or mCherry without using precious biological samples.
• Three Intensity Levels + Negative Beads
  Accommodates a range of expression levels, from dim to bright, providing greater control over compensation curves.
• Dropper Bottle Convenience
  Each test includes positive and negative populations in a single drop, simplifying setup.
• Reliable and Consistent
  Offers more reproducible results than cell-based controls, especially for unstable or heterogeneous samples.
• Compatible with All Flow Cytometers
  Designed for use across standard laser lines including 488 nm (blue), 561 nm (yellow-green), and more.

Available Kits & Fluorophore Specificity

Applications

• Flow cytometry compensation controls for fluorescent protein-expressing cells
• Multicolor panel optimization in live-cell experiments
• Transfection/transduction efficiency studies
• Quality control for engineered reporter cell lines
• Accurate gating in low-expression protein samples

Technical Specifications

Kit Contents

• 1 dropper bottle containing:
  • Three intensities of fluorescent protein-matched beads
  • Negative control beads

Invitrogen™ BrightComp eBeads™ Compensation Bead Kits are the ideal solution for compensation control in experiments involving fluorescent protein reporters. With precise spectral matching, multi-intensity options, and broad instrument compatibility, they provide a fast, accurate alternative to cell-based compensation—freeing up your samples and improving your multicolor panel performance.