Invitrogen™ Acid-Phenol: Chloroform, pH 4.5 (with IAA, 125:24:1)

Ready-to-Use Organic Extraction Reagent for High-Purity RNA Isolation

Invitrogen™ Acid-Phenol:Chloroform with Isoamyl Alcohol (IAA) is a molecular biology-grade, premixed organic extraction reagent optimized for the efficient and safe purification of total RNA. This formulation contains acid-saturated phenol, chloroform, and IAA in a 125:24:1 ratio, pre-adjusted to pH 4.5 ± 0.2, ensuring selective partitioning of RNA to the aqueous phase and DNA/proteins to the organic phase or interphase.

This ready-to-use solution removes the hazards and variability of in-lab reagent mixing while significantly improving consistency and RNA integrity in phenol-based extraction protocols. It is ideal for applications such as Northern blotting, RT-PCR, and RNA-seq.

Key Features and Benefits

• pH 4.5 for RNA-Specific Partitioning
  Acidic pH ensures efficient separation of RNA to the aqueous phase, while DNA and proteins partition into the interphase/organic phase.
• Pre-Mixed and pH-Stabilized
  Eliminates the need for in-lab preparation, improving safety, reproducibility, and convenience.
• Includes Isoamyl Alcohol (IAA)
  Helps prevent foaming, stabilize the aqueous-organic interface, and improve phase clarity during extraction.
• Molecular Biology Grade
  Certified free from RNase contamination, suitable for sensitive downstream RNA applications.
• Safer and Consistent Alternative
  Reduces exposure to hazardous crystalline phenol and avoids batch-to-batch variability.

Applications

• Total RNA isolation from cells and tissues
• Removal of DNA and protein contaminants
• Acid-phenol extraction in TRIzol™-based or guanidinium-based protocols
• Sample preparation for:
  • RT-PCR/qPCR
  • RNA-seq
  • Northern blotting
  • Microarray analysis

Technical Specifications

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 is a critical reagent for any RNA purification workflow requiring high yield and purity, with minimal DNA contamination. Trusted by researchers globally, it combines safety, convenience, and performance, making it a superior alternative to manually prepared extraction reagents.