Invitrogen™ TRIzol™ LS Reagent

The Invitrogen™ TRIzol™ LS Reagent is a complete, ready-to-use solution optimized for the isolation of high-quality RNA, DNA, and protein from liquid biological samples, including serum, plasma, and virus preparations. Its monophasic phenol and guanidine isothiocyanate formulation ensures effective lysis and preserves RNA integrity by inhibiting RNase activity during extraction. The reagent enables simultaneous isolation of RNA, DNA, and protein from a single liquid sample, streamlining molecular biology workflows.

Key Features

1. Optimized for Liquid Samples:
   • Specifically formulated for processing liquid samples like serum, plasma, and viral suspensions.
   • Handles sample volumes of up to 0.25 mL efficiently.
2. Sequential Fraction Isolation:
   • Allows extraction of RNA, DNA, and proteins from the same sample.
   • Provides clear separation of biomolecule layers during the extraction process.
3. Fast and Reliable:
   • The entire procedure is completed within 1 hour, ensuring efficient sample processing.
4. High Purity:
   • Isolated RNA is free of protein and DNA contamination.
   • Preserves RNA integrity by effectively inhibiting RNase activity during homogenization.
5. Scalable and Flexible:
   • Processes small volumes with ease, while maintaining high yields of RNA, DNA, and protein.
6. Wide Application Compatibility:
   • Isolated RNA, DNA, and protein are suitable for a wide variety of downstream applications, including:
     • qPCR
     • Northern, Southern, and Western blotting
     • NGS (Next-Generation Sequencing)
     • Molecular cloning
     • In vitro translation
     • Immunoprecipitation and enzymatic assays

Specifications

How It Works

1. Homogenization:
   • Mix 0.25 mL of liquid sample with 0.75 mL of TRIzol™ LS Reagent.
   • Homogenize to lyse cells and inhibit RNase activity.
2. Phase Separation:
   • Add chloroform and centrifuge to separate the sample into three layers:
     • Aqueous phase (RNA)
     • Interphase (DNA)
     • Organic phase (protein)
3. Sequential Precipitation:
   • Precipitate RNA with isopropanol from the aqueous phase.
   • Precipitate DNA with ethanol from the interphase.
   • Precipitate protein with isopropanol from the organic phase.
4. Washing and Resuspension:
   • Wash RNA, DNA, and protein pellets to remove impurities.
   • Resuspend purified RNA, DNA, or protein for downstream applications.

Applications

1. RNA Isolation

• Purpose:
  • Isolate high-quality RNA for use in gene expression studies.
• Use Case:
  • Perform RT-PCR, qPCR, or NGS to analyze transcriptomic profiles in serum samples.

2. DNA Isolation

• Purpose:
  • Extract DNA for genotyping or sequence analysis.
• Use Case:
  • Conduct PCR, Southern blotting, or molecular cloning with DNA isolated from liquid biopsies.

3. Protein Isolation

• Purpose:
  • Recover functional proteins for proteomic studies or enzymatic assays.
• Use Case:
  • Perform Western blotting, 2D gel electrophoresis, or enzyme recovery assays using isolated proteins.

4. Virus and Pathogen Studies

• Purpose:
  • Analyze RNA, DNA, and proteins from viral or bacterial preparations.
• Use Case:
  • Isolate viral RNA for qPCR or host proteins for immunoprecipitation from infected serum samples.

5. Multi-Omics Approaches

• Purpose:
  • Enable transcriptomic, genomic, and proteomic analyses from a single sample.
• Use Case:
  • Simultaneously study RNA, DNA, and protein interactions in serum-derived biomarkers.

Advantages

1. Streamlined Process:
   • Facilitates the simultaneous isolation of multiple biomolecules, reducing the need for separate extraction kits.
2. High Yield and Purity:
   • Produces RNA, DNA, and protein free of contaminants, ensuring reliable downstream results.
3. Time Efficiency:
   • Complete the entire procedure in 1 hour, saving valuable lab time.
4. Versatile Application Range:
   • Compatible with a wide variety of biological fluids and downstream molecular techniques.
5. Scalable:
   • Processes small liquid volumes effectively, making it suitable for rare or limited samples.
6. Stability:
   • The reagent is stable at room temperature, making storage and handling convenient.

Use Cases by Research Area

Comparison of Invitrogen™ TRIzol™ LS Reagent vs. Invitrogen™ TRIzol™ Reagent

Summary of Key Differences

1. Target Sample Type:
   • TRIzol™ LS Reagent is optimized for liquid samples (e.g., serum, plasma, and viral preparations).
   • TRIzol™ Reagent is designed for solid and liquid samples, including tissues and cultured cells.
2. Concentration:
   • TRIzol™ LS Reagent has a higher concentration to accommodate larger liquid sample volumes.
3. Yield:
   • RNA yields from TRIzol™ Reagent are generally higher for tissue samples.
   • TRIzol™ LS Reagent provides optimized yields for RNA extraction from liquid samples.
4. Applications:
   • Both reagents are versatile but tailored to different starting materials.

Storage and Handling

1. Storage:
   • Store at room temperature in its original container.
2. Handling:
   • Avoid exposure to direct sunlight and ensure proper ventilation when handling phenol-containing reagents.
3. Safety Precautions:
   • Follow standard laboratory safety procedures when working with phenol-based reagents.

The Invitrogen™ TRIzol™ LS Reagent provides a robust, scalable, and versatile solution for extracting RNA, DNA, and protein from liquid samples, making it an invaluable tool for researchers in molecular biology, genomics, and proteomics. Let me know if you need additional details or assistance!