Gibco™ TrypLE™ Select Enzyme (10X), no phenol red

Gibco™ TrypLE™ Select Enzyme (10X), No Phenol Red, is a highly pure, animal origin-free (AOF), recombinant enzyme designed for dissociation of a wide range of adherent mammalian cells. This 10X concentrated formulation offers convenience, flexibility, and superior performance compared to traditional trypsin-based dissociation reagents. It is gentle on cells, ensuring higher viability and reproducibility in cell culture workflows.

Key Features:

1. Gentle on Cells:
   • Maintains cell health by cleaving peptide bonds with minimal damage, supporting higher viability and reproducibility.
2. Concentrated (10X):
   • Can be diluted aseptically to a 1X working solution using a balanced salt solution containing 1 mM EDTA.
3. Room-Temperature Stable:
   • Remains stable for 24 months at 15–30°C, allowing flexible storage and handling.
4. Animal Origin-Free (AOF):
   • Manufactured on dedicated animal-free equipment, ensuring purity and suitability for regulatory-compliant workflows.
5. No Phenol Red:
   • Ideal for applications requiring phenol red-free conditions to avoid interference.
6. Self-Inactivating:
   • Inactivated by simple dilution, eliminating the need for trypsin inhibitors like FBS.

Specifications:

Applications:

1. Adherent Mammalian Cell Culture:
   • Efficiently dissociates adherent cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells.
2. Primary Cell Isolation:
   • Suitable for gentle dissociation of tissues into single-cell suspensions for downstream experiments.
3. Cell Passaging:
   • Ideal for routine subculturing of sensitive or high-value adherent cell lines.
4. Regulatory-Compliant Research:
   • AOF formulation meets stringent regulatory and manufacturing requirements.
5. High-Throughput Workflows:
   • Concentrated format supports scalability for large-scale experiments.

Advantages:

1. High Purity:
   • Reduces cell damage caused by nonspecific cleavage from multiple enzymes present in trypsin extracts.
2. Regulatory Compliance:
   • Manufactured under cGMP conditions, certified to ISO 13485, and registered with the FDA.
3. Flexible Storage:
   • Stable across a range of temperatures, reducing storage and handling limitations.
4. Easy Integration:
   • Directly replaces trypsin in existing protocols without modification.
5. Eco-Friendly Manufacturing:
   • Produced using energy-efficient processes and sustainable packaging.

Protocol Overview:

1. Prepare TrypLE™ Solution:
   • Dilute TrypLE™ Select 10X to 1X with a balanced salt solution containing 1 mM EDTA.
2. Wash Cells:
   • Wash adherent cells with PBS or equivalent to remove serum that may inhibit enzyme activity.
3. Apply TrypLE™:
   • Add the diluted TrypLE™ solution to the cell culture to cover the monolayer.
4. Incubate:
   • Incubate at 37°C for 2–5 minutes, monitoring for cell detachment.
5. Neutralize:
   • Dilute TrypLE™ Select with complete growth medium to inactivate the enzyme.
6. Centrifuge and Resuspend:
   • Centrifuge cells and resuspend in fresh medium for further use.

Ordering Information:

Storage and Handling:

• Storage Conditions:
  • Store at 15–30°C. For extended stability, store at 2–8°C or -5°C to -20°C.
• Shelf Life:
  • 24 months from the date of manufacture.

Related Products:

• Gibco™ TrypLE Express (1X): For immediate use without dilution.
• Gibco™ DMEM/F-12 Medium: For culturing cells post-dissociation.
• Gibco™ PBS Without Calcium and Magnesium: For pre-washing cells before dissociation.

Product Applications and Benefits:

1. Primary Cell Research:
   • Supports gentle isolation of primary cells for precise downstream experiments.
2. Bioprocessing:
   • Animal origin-free formulation ensures compliance for manufacturing processes.
3. Stem Cell Studies:
   • Maintains stem cell viability during dissociation and passaging.
4. Eco-Friendly Research:
   • Sustainable and efficient manufacturing reduces environmental impact.

The Gibco™ TrypLE™ Select Enzyme (10X), No Phenol Red, offers a reliable, high-performance solution for cell dissociation, with superior purity, regulatory compliance, and flexibility. Its concentrated format provides scalability, making it an excellent choice for a wide range of cell culture applications.