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Invitrogen™ Glutathione Colorimetric Detection Kit, 4 x 96 Tests
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Quantitative colorimetric assay for measuring total and oxidized glutathione in diverse biological samples
The Invitrogen™ Glutathione Colorimetric Detection Kit is a ready-to-use, research-use-only assay for quantitative measurement of total glutathione (GSH + GSSG), free GSH, and oxidized glutathione (GSSG) in whole blood, plasma, serum, erythrocytes, urine, cell lysates, and tissue samples. This species-independent assay features a simple, no-wash workflow and delivers sensitive, reproducible results using a microplate reader at 405–412 nm.
Glutathione is the most abundant non-protein thiol in mammalian cells, playing vital roles in redox homeostasis, detoxification, protein and DNA synthesis, and cellular defense against oxidative stress. Accurate quantification of GSH and GSSG is essential for oxidative stress, metabolism, and toxicology research.
Key Features
- High Sensitivity – Detects glutathione concentrations as low as 0.634 μM
- Wide Dynamic Range – 0.78–25 μM standard curve range
- No Separation or Wash Steps – Rapid, user-friendly workflow
- Total, Free, and Oxidized GSH Quantification – Uses 2-vinylpyridine treatment to distinguish GSSG from total GSH
- Versatile Sample Compatibility – Works with a wide range of biological fluids and extracts
- Reproducible – Low intra- and inter-assay variability
Applications
- Oxidative stress biomarker measurement
- Redox status profiling in cells and tissues
- Metabolic pathway analysis involving glutathione
- Toxicology studies assessing oxidative damage
- Antioxidant therapeutic evaluation
- Disease biomarker research for neurodegenerative, cardiovascular, and metabolic disorders
Assay Principle
- Total GSH Measurement – The free thiol group of GSH reacts with the kit’s colorimetric substrate to form a highly colored product measured at 405 nm.
- GSSG Measurement – 2-vinylpyridine treatment blocks free GSH, allowing selective measurement of oxidized GSSG.
- Free GSH Calculation – Obtained by subtracting GSSG (converted to GSH equivalents) from total GSH.
Results can be obtained via endpoint detection or by kinetic measurement of color development.
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | EIAGSHC |
| Detection Method | Colorimetric (Absorbance at 405–412 nm) |
| Sensitivity | 0.634 μM |
| Standard Curve Range | 0.78–25 μM |
| Sample Types | Whole blood, plasma, serum, erythrocytes, urine, cell lysates, tissue samples |
| Assay Format | 4 × 96-well plates |
| Storage Conditions | –20°C |
| Shipping Conditions | Wet or dry ice |
| Regulatory Status | Research Use Only (RUO) |
The Invitrogen™ Glutathione Colorimetric Detection Kit offers a sensitive, accurate, and easy-to-use method for quantifying glutathione in a wide range of biological samples. Its ability to measure total, free, and oxidized GSH makes it an essential tool for oxidative stress, redox biology, and antioxidant research.