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Invitrogen™ Lactate Dehydrogenase (LDH) Colorimetric Activity Kit, 96 Tests
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Lactate Dehydrogenase (LDH) Colorimetric Activity Kit is a highly sensitive, reliable, and easy-to-use assay for the quantitative measurement of LDH activity in serum, plasma, tissue homogenates, cell culture supernatants, whole cells, and hydrothorax samples.
LDH is a key enzyme in cellular energy metabolism, catalyzing the conversion of lactate to pyruvate. Elevated LDH levels are well-established biomarkers for tissue damage, inflammation, cancer progression, and metabolic disorders. This kit is ideal for clinical research, toxicology testing, metabolic pathway analysis, and disease biomarker studies.
Now with free access to our online LDH Colorimetric Activity Kit Calculator, you can quickly and accurately calculate LDH activity from your OD readings. Simply input your absorbance values, and the calculator automatically generates results using your standard curve — reducing analysis time and improving data reliability.
Assay Principle
In this colorimetric assay, LDH catalyzes the oxidation of lactic acid to pyruvate. Pyruvate reacts with 2,4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, a red-brown complex in alkaline solution. The intensity of the color, measured with a microplate reader, is directly proportional to LDH activity in the sample.
Key Features
- Free LDH Calculator – Eliminates manual calculations and speeds up data analysis.
- High Sensitivity – Detects as low as 6 U/L LDH activity.
- Fast & Efficient – Total assay time of 45 minutes with minimal hands-on steps.
- Broad Sample Compatibility – Works with serum, plasma, hydrothorax, tissue homogenates, and whole cells.
- Reproducible Results – Low intraassay CV (1.8%) and interassay CV (2.4%).
- User-Friendly Protocol – Simple colorimetric readout for easy interpretation.
Applications
- Biomarker Studies – Detect LDH release as an indicator of tissue damage or inflammation.
- Cancer Research – Track LDH activity as a marker for tumor metabolism.
- Cytotoxicity Testing – Evaluate cell damage caused by drugs or toxins.
- Metabolic Pathway Analysis – Measure LDH activity in energy metabolism studies.
- Cardiac & Liver Research – Monitor LDH levels in cardiovascular and hepatic disease models.
Technical Specifications
| Parameter | Specification |
|---|---|
| Assay Range | 6–1000 U/L |
| Assay Sensitivity | 6 U/L |
| Detection Method | Colorimetric |
| Total Assay Time | 45 minutes |
| Incubation Time | 35 minutes |
| Sample Volume | 40 μL |
| Sample Types | Serum, Plasma, Hydrothorax, Tissue Homogenates, Whole Cells |
| Intraassay CV | 1.8% |
| Interassay CV | 2.4% |
| For Use With | Microplate Reader |
| Storage | 2–8°C |
| Shipping Condition | Dry Ice, 2–8°C |
| Unit Size | Each (96 tests) |
Kit Contents
- Assay Buffer
- 2,4-Dinitrophenylhydrazine Reagent
- Lactic Acid Substrate
- Pyruvate Standard
- Detailed Protocol
The Invitrogen™ Lactate Dehydrogenase (LDH) Colorimetric Activity Kit, now paired with a free online LDH Calculator, delivers fast, sensitive, and reproducible results for LDH quantification. This combination streamlines both experimental workflow and data processing, making it an essential tool for biomarker research, toxicology studies, and metabolic enzyme analysis.