Thermo Scientific™ Modified Semi-Solid Rappaport-Vassiliadis (MRSV) Medium Base (Dehydrated)

Thermo Scientific™ Oxoid™ Modified Semi-Solid Rappaport-Vassiliadis (MSRV) Medium Base is a selective and differential medium designed for the isolation of motile Salmonella species from food and environmental samples. The medium enhances motility-based enrichment, allowing Salmonella to migrate through the medium ahead of competing microorganisms and form distinct halos of growth.

Key Features

1. Selective Medium for Salmonella

• Inhibits Gram-positive organisms with:
  • Novobiocin
  • Low pH
  • High magnesium chloride concentration
  • Malachite green

2. Motility-Based Isolation

• Utilizes the motility of Salmonella to migrate through the medium ahead of non-Salmonella competitors, forming opaque halos of growth, enabling sensitive and specific isolation.

3. Enhanced Sensitivity

• Designed to detect more Salmonella-positive samples compared to traditional enrichment procedures, as validated by multiple studies.

4. Versatile Applications

• Suitable for:
  • Food testing
  • Environmental monitoring (e.g., dust in animal production facilities)

Applications

1. Food Microbiology:
   • Isolate motile Salmonella from contaminated food products.
2. Environmental Monitoring:
   • Detect Salmonella in animal production environments (dust, feed, etc.).
3. Public Health Surveillance:
   • Enhance sensitivity in outbreak investigations.
4. Veterinary Microbiology:
   • Screen for Salmonella in livestock and animal production facilities.

Composition (Typical Formula)

Preparation Instructions

1. Suspend 31.5g of dehydrated medium in 1 litre of distilled water.
2. Heat to dissolve the medium completely.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45–50°C, mix thoroughly, and pour into sterile Petri dishes.

Advantages

1. Enhanced Detection:
   • Detects more Salmonella-positive samples than traditional methods.
2. Selective and Sensitive:
   • Efficiently suppresses non-target organisms while enhancing Salmonella motility.
3. Ease of Use:
   • Simple preparation and easy-to-interpret results with distinct growth halos.
4. Wide Applicability:
   • Suitable for both food and environmental microbiology.

Quality Control

Specifications

• Form: Powder
• Yield: 500g makes approximately 15.8L of medium
• Certifications/Compliance: NMKL
• Unit Size: Each

Storage and Shelf Life

1. Store the dehydrated medium at 10–30°C in a tightly sealed container.
2. Use before the expiration date printed on the label.

Why Choose Thermo Scientific™ Oxoid™ MSRV Medium Base?

The Modified Semi-Solid Rappaport-Vassiliadis (MSRV) Medium Base is an advanced, selective medium optimized for Salmonella isolation using motility-based enrichment. Its superior sensitivity and inhibitory properties make it a reliable choice for laboratories focused on food safety, environmental testing, and public health investigations.