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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
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Specifications:
| Application | qPCR | ||
| Storage Temperature | 2-8°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
PowerUp™ SYBR™ Green Master Mix is a universal 2X qPCR master mix designed for high specificity, enhanced reproducibility, and superior performance across a wide range of gene expression studies. It features Dual-Lock™ hot-start Taq DNA polymerase and heat-labile UDG for carryover prevention. Its consistent performance allows reaction setup in advance (up to 72 hours before cycling), making it ideal for high-throughput and automated workflows.
Key Features
- ✅ Dual hot-start technology (antibody and chemical modifications) provides precise control and prevents premature polymerase activity
- ✅ Compatible with fast and standard cycling protocols
- ✅ Includes ROX passive reference dye for instrument normalization
- ✅ Contains UNG (UDG) and dUTP to prevent carryover contamination
- ✅ Demonstrated tight CT reproducibility across 6-log dynamic range
- ✅ Validated for use with Applied Biosystems, Bio-Rad, Roche, and Agilent qPCR instruments
Use Cases
🧬 Gene Expression Analysis
- Accurate quantification of target genes in various tissue types
🦠 Pathogen Detection / Viral Load Quantification
- Reliable Ct values over a wide input range, especially useful in diagnostic research settings
🧪 Low-Copy Target Detection
- Enhanced specificity minimizes background signal, critical for detecting rare transcripts
📦 High-Throughput Screening
- Pre-assembled reactions are stable for up to 72 hours, allowing batch setup and automation
✅ Comparison: PowerUp™ vs. PowerTrack™ SYBR Green Master Mix
| Feature | PowerUp™ SYBR Green Master Mix | PowerTrack™ SYBR Green Master Mix |
|---|---|---|
| Enzyme System | Dual-Lock™ hot-start Taq (antibody + chemical) | Antibody-mediated hot-start Taq |
| Visual Dye Indicators | ❌ None | ✅ Blue master mix + optional yellow buffer (turns green when combined) |
| Carryover Contamination Control | ✅ Includes UDG/dUTP | ✅ Includes UNG/dUTP |
| Pre-Assembled Reaction Stability | ✅ Up to 72 hours at room temperature before cycling | ❌ Not specified |
| Reproducibility | ✅ Tight CTs, 6-log dynamic range | ✅ Consistent CTs across a broad dynamic range |
| Optimization Requirements | Low (broad primer Tm and concentration compatibility) | Minimal optimization needed |
| Visual Setup Confirmation | ❌ Not available | ✅ Color change ensures pipetting accuracy |
| Instrument Compatibility | Applied Biosystems™, Bio-Rad™, Roche™, Agilent™ | Applied Biosystems™, Bio-Rad™, Roche™, Agilent™ |
| Best For | ✅ High-throughput, automation, gene expression, contamination-sensitive workflows | ✅ Beginner-friendly, visual confirmation, educational labs, qPCR newbies |
Recommendations by Use Case
| Application | Best Choice | Why |
|---|---|---|
| Routine gene expression (qPCR) | PowerUp™ | Higher reproducibility, batch setup stability |
| Educational/teaching labs | PowerTrack™ | Visual pipetting confirmation (color change) |
| Carryover contamination-prone labs | Both | Both contain UNG/UDG and dUTP |
| First-time qPCR users | PowerTrack™ | Easy setup with color-coded reagents |
| High-throughput labs (automation) | PowerUp™ | Stable pre-assembled reactions for up to 72 hours |
| Low-expressing gene detection | PowerUp™ | Superior enzyme control with dual hot-start system |
For Research Use Only. Not for use in diagnostic procedures.
- Pack Size: 1 x 1 mL, sufficient for 100 Rxns 1 x 5 mL, sufficient for 500 Rxns 2 x 5 mL, sufficient for 1000 Rxns 5 x 5 mL, sufficient for 2500 Rxns 10 x 5 mL, sufficient for 5000 Rxns 1 x 50 mL, sufficient for 5000 Rxns 5 x 1 mL, Sufficient for 500 Rxns 10 x 1 mL, Sufficient for 1000 Rxns 2 x 1 mL, Sufficient for 200 Rxns