Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

The Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix is a pre-formulated, universal 2X qPCR master mix designed for high-performance real-time PCR (qPCR) and gene expression analysis.

Featuring Dual-Lock™ Taq DNA Polymerase and UDG-based contamination control, this mix provides exceptional specificity, reproducibility, and stability, making it ideal for researchers performing quantitative gene expression, genotyping, and DNA quantification.

This ready-to-use mix supports both standard and fast cycling protocols and is fully compatible with Applied Biosystems™ QuantStudio™, StepOne™, 7500, 7900HT, and ViiA™ 7 systems, as well as other major qPCR instruments.

Key Features

• Dual hot-start enzyme activation (Dual-Lock™ Taq DNA Polymerase): Ensures superior specificity by controlling enzyme activity at lower temperatures.
• Broad dynamic range: Delivers reproducible CT values over six logs of sample input.
• Incorporated UDG system: Prevents carryover contamination from previous PCR products.
• Flexible workflow: Assembled reactions remain stable for up to 72 hours before cycling.
• Universal instrument compatibility: Works with all Applied Biosystems™ qPCR platforms and most third-party systems (Bio-Rad™, Roche™, Agilent™).
• ROX reference dye pre-mixed: Eliminates the need for manual dye addition.

Technical Specifications

Applications

• Quantitative PCR (qPCR): Gene expression profiling and quantification of DNA or cDNA.
• Gene Expression Analysis: Evaluate relative or absolute gene expression across biological samples.
• Genotyping and Validation: High-specificity amplification of target sequences.
• Contamination-free assays: Suitable for UDG-enabled carryover prevention workflows.
• Molecular Biology Research: Quantitative amplification for molecular diagnostics, research, and development.

Unique Advantages

• Dual hot-start specificity control: Prevents non-specific amplification and primer-dimer formation.
• Exceptional reproducibility: Consistent CT values across multiple runs and templates.
• Stable pre-assembled reaction capability (72 h): Flexible setup for high-throughput workflows.
• Universal instrument compatibility: One mix for all SYBR-based qPCR platforms.
• Pre-mixed passive reference dye (ROX): Simplifies workflow and reduces variability.
• Built-in UDG system: Ensures PCR integrity by removing carryover amplicons.

Ordering Information

Storage & Stability

• Store at 2–8°C, protected from light.
• Do not freeze.
• Stable for up to 72 hours post-assembly at room temperature prior to cycling.

The Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix provides superior specificity, sensitivity, and reproducibility for SYBR-based real-time PCR applications.

With Dual-Lock™ Taq polymerase, built-in UDG system, and compatibility across all major qPCR instruments, this mix simplifies qPCR workflows and ensures reliable, contamination-free amplification.

An ideal choice for gene expression studies, quantification assays, and routine qPCR applications in molecular biology research.