0
Merck Enterococcus Selective Agar suitable for microbiology, NutriSelect® Basic
Catalog No :
CAS Number :
Brand :
In Stock
Selective Medium for Enterococci & Group D Streptococci
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Millipore | ||
| Product Grade | Microbiology grade | ||
Merck Enterococcus Selective Agar, also known as Slanetz and Bartley Agar or m-Enterococcus Agar, is a selective and differential culture medium designed for the enumeration and detection of enterococci in water, sewage, food, and environmental samples. This formulation complies with ISO 7899-2:2000, making it suitable for standardized water quality monitoring and bioburden assessments.
The medium supports the growth of Enterococcus spp. and Group D streptococci, enabling clear differentiation through color reactions mediated by 2,3,5-triphenyltetrazolium chloride (TTC). Enterococcal colonies typically appear red, maroon, or pink due to TTC reduction, aiding rapid and reliable identification.
Ordering Information
| Pack Size | SKU |
|---|---|
| 500 g | 45183-500G |
Key Features
- Selective and differential medium for Enterococcus spp.
- Complies with ISO 7899-2:2000 for water analysis.
- Suitable for membrane filtration and direct plating methods.
- Ideal for bioburden testing, food microbiology, environmental monitoring, and clinical testing.
- Powdered NutriSelect® Basic format (cost-efficient option).
- Clear chromogenic-like reaction using TTC for reliable colony differentiation.
Composition (per Liter)
- Tryptose — 20 g
- Agar — 10 g
- Yeast extract — 5 g
- Disodium hydrogen phosphate monohydrate — 4 g
- D(+)-Glucose — 2 g
- Sodium azide — 0.4 g
- 2,3,5-Triphenyltetrazolium chloride (TTC) — 0.1 g
This composition ensures selectivity against Gram-negative organisms while supporting robust growth of enterococci.
Properties & Specifications
| Parameter | Details |
|---|---|
| Quality Level | 200 |
| Form | Powder |
| Sterility | Non-sterile |
| Final pH | 7.2 ± 0.2 at 25°C |
| Shelf Life | Limited; see label |
| Brand | NutriSelect® Basic |
| Technique | Suitable for microbiological culture |
| Suitability | Selective & differential for Enterococcus spp. and Streptococcus spp. |
| UNSPSC Code | 41171606 |
| NACRES | NA.85 |
Applications
- Water monitoring (ISO 7899-2 membrane filtration)
- Environmental microbiology
- Bioburden and contamination testing
- Food and beverage quality control
- Clinical and diagnostic microbiology
- Detection of enterococci in sewage, surface water, drinking water, and food samples
Also cited for use in enumeration of sulphite-reducing Clostridium spores and differentiation of Group D faecal streptococci in oysters.
Preparation Note
- Dissolve 42 g of medium in 1 L of distilled water.
- Sterilize in a steamer without pressure for 30 minutes.
- Do not autoclave.
- Pour plates.
This gentle sterilization preserves medium selectivity and TTC reaction integrity.
Additional Notes
- Available in both GranuCult® (granulated, superior quality) and NutriSelect® (powdered, economical) formats.
- Quality designations: Basic, Plus, Prime, depending on QC and regulatory compliance level.
Merck Enterococcus Selective Agar (NutriSelect® Basic) is a reliable, ISO-compliant medium for detection and enumeration of enterococci across water, environmental, clinical, and food-testing laboratories. Its proven selectivity, clear visual colony differentiation, and standard preparation protocol make it a trusted choice for routine microbiological surveillance and regulatory testing.