Invitrogen™ Goat anti-Human IgM Secondary Antibody, HRP

The Invitrogen™ Goat anti-Human IgM Secondary Antibody, HRP-conjugated (Catalog #31415) is a polyclonal, affinity-purified antibody specifically designed for the detection of human IgM (Fc5μ fragment) in immunoassays. This antibody is conjugated to Horseradish Peroxidase (HRP) to enable enzyme-based signal detection in applications such as Western blot (WB), Immunohistochemistry (IHC), and Immunocytochemistry/Immunofluorescence (ICC/IF).

With exceptional sensitivity, minimal cross-reactivity, and broad dynamic range, this secondary antibody is ideal for detecting human IgM heavy chains in complex protein mixtures using colorimetric or chemiluminescent detection systems.

Key Features & Benefits

✅ Specific for Human IgM Heavy Chain (Fc5μ Region)

• Selectively binds the Fc5μ portion of human IgM.
• Demonstrated no cross-reactivity with human IgG or IgA in electrophoresis-based testing.
• May cross-react with IgM from other species—validate if needed.

✅ Conjugated to HRP for Enzyme-Based Detection

• Enables colorimetric, chemiluminescent, or fluorescent detection using compatible substrates (e.g., TMB, DAB, ECL).

✅ Affinity-Purified for High Specificity

• Purified using affinity chromatography for low background and high signal clarity.

✅ Lyophilized for Long-Term Stability

• Supplied lyophilized; reconstitute with 2.0 mL distilled water to yield 0.8 mg/mL working concentration.
• Extended shelf life when stored properly.

Applications

Product Specifications

Restoration & Storage Instructions

1. Reconstitution: Add 2.0 mL of distilled water to lyophilized vial to obtain a final concentration of 0.8 mg/mL.
2. Allow the solution to stand 1–2 hours at room temperature, then centrifuge if solution is not clear.
3. Short-term storage: Store undiluted at 4°C for several weeks.
4. Long-term storage: Add an equal volume of glycerol (final 50%) and store at –20°C.
5. Post-dilution usage: Use immediately—do not store diluted antibody for extended periods.

Target Information – Human IgM

• Immunoglobulin M (IgM) is the first antibody isotype produced during an immune response and plays a vital role in early immunity.
• It exists primarily in a pentameric form in the bloodstream and is critical in neutralizing pathogens, activating complement, and clearing antigens.
• The Fc5μ portion is a unique region of the IgM heavy chain, enabling selective detection of IgM without cross-reactivity to IgG or IgA.

Compatible Detection Systems

• HRP Substrates:
  • TMB (3,3′,5,5′-Tetramethylbenzidine) for ELISA
  • DAB (3,3′-Diaminobenzidine) for IHC
  • ECL (Enhanced Chemiluminescence) for Western blot
• Compatible with a variety of membranes (PVDF, nitrocellulose) and imaging systems.

Why Choose This Antibody?

✅ Highly specific to human IgM (Fc5μ)—no cross-reactivity with IgG or IgA

✅ Versatile across multiple applications: WB, IHC, ICC/IF

✅ HRP conjugation supports sensitive enzymatic detection methods

✅ Reliable performance in immunoassays with low background noise

✅ Backed by Invitrogen quality and validation data

This antibody is ideal for researchers studying primary immune responses, B cell activity, autoimmune conditions, and human IgM protein expression using HRP-based detection.