Invitrogen™ 123count eBeads™ Counting Beads, 100 Tests

Absolute Cell Counting Beads for Flow Cytometry with Blue and Violet Laser Compatibility

123count eBeads™ are precision-formulated microparticles designed for absolute quantification of cells or particles by flow cytometry. These 7 μm fluorescent beads are optimized for use with standard blue (488 nm) and violet (405 nm) lasers and emit across a broad range of 500–750 nm, ensuring compatibility with a wide variety of cytometers and fluorochromes.

Each batch of beads is supplied at a known concentration in a stabilized solution, allowing for consistent and reproducible ratiometric enumeration of cells in suspension without the need for additional counting devices.

Key Features and Benefits

• Reliable Absolute Counting
  Enables accurate enumeration of cells or particles based on bead-to-cell ratios collected by flow cytometry.
• Laser Compatibility
  Fluorescent dyes are excited by 405 nm and 488 nm lasers, emitting from ~500–750 nm for broad detection.
• Consistent and Reproducible
  Supplied at a known concentration in 0.05% Tween-20 and 2 mM sodium azide for stable and consistent use.
• 7 μm Bead Size
  Ideal for side scatter and gating alongside common cell populations in suspension.
• Flexible and Easy to Use
  Simply mix a known volume of sample with beads and analyze using your flow cytometer.

Applications

• Absolute cell counting in flow cytometry
• Enumeration of rare cell subsets (e.g., stem cells, Tregs)
• Standardizing cell input for downstream assays
• Determining cell concentrations in PBMC or whole blood samples
• Bead-based flow cytometric quality control

Technical Specifications

Invitrogen™ 123count eBeads™ Counting Beads are an essential tool for accurate, reproducible absolute cell counting in flow cytometry. With broad laser compatibility, stable formulation, and a simple workflow, they ensure confidence in cell quantification across a wide range of research applications.