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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreen)
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Reading Options: Top and Bottom
No. of dispensers: 1
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader delivers unmatched performance and flexibility for bioscience and drug-discovery laboratories. This modular system integrates five advanced detection technologies—Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence (TRF), and AlphaScreen/AlphaLISA—with precise environmental control for cell-based assays.
Equipped with an integrated Gas Module and one onboard dispenser, the Varioskan™ LUX (VLBLATGD1) enables simultaneous gas regulation and reagent addition for kinetic reactions. Smart safety systems, automated calibration, and SkanIt™ Software (Research Edition) simplify operation, ensuring data reliability and complete traceability.
Key Features
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Five measurement technologies in a single compact platform:
Absorbance (UV–Vis), Fluorescence Intensity (incl. FRET), Luminescence (direct & filtered), AlphaScreen/AlphaLISA, and Time-Resolved Fluorescence (incl. TR-FRET/hTRF). - Integrated Gas Module for CO₂ (0.1–15 %) and O₂ (1–21 %) control—ideal for live-cell and hypoxia studies.
- Built-in dispenser (1) supporting 2–5 000 µL volumes (1 µL increments) with simultaneous measurement.
- Automatic dynamic range selection for optimal signal acquisition without manual adjustment.
- Spectral scanning for wavelength optimization across 200–1000 nm.
- Smart Safety Controls and real-time diagnostics to prevent setup errors.
- Modular, upgradeable design for future expansion.
- HTRF™ Certified by Cisbio Bioassays and DLReady™ Validated by Promega Corporation.
SkanIt™ Software Highlights (Research Edition Included)
- License-free installation—each user may install on their workstation.
- Virtual Pipetting Tool for rapid plate-layout definition.
- Real-time visualization of kinetic curves and spectra.
- Continuous autosave safeguards data against unexpected interruptions.
- Built-in data processing: blank subtraction, SD/CV %, dose-response curves, kinetic/spectral analyses, QC criteria, and custom formulas.
- Automated export to *.xlsx, *.pdf, *.xml, *.txt formats or automatic email delivery after run completion.
- Comprehensive audit-ready reporting for traceability.
- Optional Drug Discovery Edition supports FDA 21 CFR Part 11 compliance.
Applications
- Cell-based assays under controlled CO₂/O₂ conditions
- Enzyme kinetics and signal-onset monitoring
- Reporter-gene and luminescence assays
- FRET, TR-FRET (hTRF), AlphaScreen/AlphaLISA
- Quantitative ELISA, nucleic-acid and protein assays
- High-throughput compound screening
- Spectral and kinetic scanning for assay development
Technical Specifications
| Parameter | Specification |
|---|---|
| Catalog Number | VLBLATGD1 |
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence, AlphaScreen |
| Reading Options | Top and Bottom |
| Dispensers | 1 (2 – 5 000 µL, 1 µL increments) |
| Gas Control Module | CO₂ 0.1–15 %, O₂ 1–21 % |
| Wavelength Range | Abs 200–1000 nm; FI Exc. 200–822 nm / Em. 270–840 nm; TRF Exc. 334 nm / Em. 400–700 nm; Lum 360–670 nm; AlphaScreen Exc. 680 nm / Em. 400–660 nm |
| Spectral Scanning Speed | < 2.2 s/well (400–500 nm, 2 nm step) |
| Measurement Modes | Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | FI > 6 decades (top), Lum > 7 decades, TRF > 6 decades |
| Sensitivity | FI (top) < 0.4 fmol fluorescein/well; FI (bottom) < 4 fmol; TRF < 1 amol/well; Lum < 7 amol ATP/well; AlphaScreen < 100 amol phosphotyrosine/well |
| Photometric Accuracy | 0.003 Abs or ±2 % (200–399 nm, 0–2 Abs); ±1 % (400–1000 nm, 0–3 Abs) |
| Linearity | ±2 % (96-well 0–4 Abs @ 450 nm; 384-well 0–3 Abs) |
| Precision | SD < 0.001 Abs or CV < 0.5 % (450 nm, 0–3 Abs) |
| Plate Compatibility | 6 – 1536 wells (Abs 6–384 wells); μDrop plates |
| Incubator Temperature | Ambient + 4 °C to 45 °C |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp and LED |
| Shaking | Orbital |
| Dimensions (L × W × H) | 58 × 53 × 51 cm (23 × 21 × 20 in.) |
| Weight | 57 kg (125 lb) |
| Interface | Thermo Scientific™ SkanIt™ Software |
| Product Line | Varioskan™ |
| Read-out Range | 0 – 6 Abs |
| Included Accessories | Gas Module, SkanIt PC Software (Research Edition), Nunc™ 96-Well Optical-Bottom Microplate (black, TC surface) |
Unique Advantages
- Gas-regulated environment for true physiological assay conditions.
- Simultaneous dispense-and-read function captures transient kinetics instantly.
- Unparalleled detection sensitivity across all modes.
- Future-proof design supporting modular upgrades and automation integration.
- Validated by industry leaders (HTRF™ / DLReady™) ensuring compatibility with major assay platforms.
Ordering Information
| Catalog Number | Measurement Technologies | Reading Options | Dispensers | Includes |
|---|---|---|---|---|
| VLBLATGD1 | Absorbance, Fluorescence Intensity, Luminescence, TRF, AlphaScreen | Top and Bottom | 1 | Gas Module and SkanIt PC Software (Research Edition) |
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBLATGD1) represents the next generation of microplate detection—combining spectral versatility, precise environmental control, and intuitive automation. Its integrated gas module and dispenser enable complex cell-based and kinetic assays with reproducible accuracy. Ideal for researchers seeking flexibility, traceability, and trusted results across life science and drug discovery applications.