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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreen)
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Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBLATD2) is a high-performance, modular instrument designed to meet the demanding requirements of life science, drug discovery, and biochemical research laboratories.
Combining up to five powerful measurement technologies—Absorbance (UV-Vis), Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence (TRF), and AlphaScreen/AlphaLISA—the Varioskan™ LUX delivers exceptional precision, sensitivity, and flexibility for a wide range of microplate-based assays.
This configuration includes two built-in dispensers for precise and rapid reagent addition, enabling real-time kinetic measurements and reaction monitoring from the very start. With features such as automatic dynamic range selection, spectral scanning, and smart safety controls, the Varioskan™ LUX simplifies workflows, minimizes errors, and ensures reproducible data across applications.
Key Features
- Five measurement technologies: Absorbance (UV-Vis), Fluorescence Intensity (including FRET), Luminescence (direct and filtered), AlphaScreen/AlphaLISA, and Time-Resolved Fluorescence (including TR-FRET/hTRF).
- Dual dispensers (2): Programmable channels (2 – 5 000 µL, 1 µL increments) for simultaneous or sequential reagent addition during kinetic assays.
- Automatic Dynamic Range Selection: System automatically adjusts measurement sensitivity to avoid saturation and ensure accurate quantification.
- Spectral Scanning: Rapid wavelength scanning (< 2.2 s/well, 2 nm step) for assay optimization and spectral profiling.
- Smart Safety Controls: Built-in diagnostic features detect potential setup issues before measurement begins.
- HTRF™ Certified by Cisbio Bioassays and DLReady™ Validated by Promega Corporation, ensuring broad assay compatibility.
- Modular and upgradeable design: Expandable system architecture allows additional modules and accessories.
- Seamless automation compatibility: Integrates with robotic handlers and automated laboratory workflows.
- SkanIt™ Software included: Intuitive, license-free platform for setup, data acquisition, and analysis.
SkanIt™ Software (Research Edition Included)
- License-free installation for unlimited users.
- Virtual Pipetting Tool for simplified plate layout definition.
- Real-time visualization of spectra and kinetic curves during acquisition.
- Continuous autosave ensures no data loss due to power or software interruptions.
- Built-in analysis tools: blank subtraction, SD/CV%, standard curve, dose-response, quality control, kinetic and spectral analysis, and custom formula creation.
- Flexible export formats: *.xlsx, *.pdf, *.xml, *.txt — or automatic email export after run completion.
- Traceable reporting with complete runtime documentation.
- Optional Drug Discovery Edition available for FDA 21 CFR Part 11 compliance.
Applications
- Enzyme kinetics and reaction onset studies
- FRET/TR-FRET and AlphaScreen/AlphaLISA assays
- Reporter gene and ATP-based luminescence assays
- ELISA, DNA/RNA quantification, and protein concentration analysis
- Spectral scanning for dye optimization and multiplexing
- Cell-based assays and biochemical reaction monitoring
- Drug discovery and high-throughput screening
Technical Specifications
| Parameter | Specification |
|---|---|
| Catalog Number | VLBLATD2 |
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence, AlphaScreen |
| Reading Options | Top and Bottom |
| Dispensers | 2 (2 – 5 000 µL, 1 µL increments) |
| Measurement Modes | Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra |
| Spectral Scanning Speed | < 2.2 s/well (400–500 nm, 1 flash, 2 nm step) |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | FI: top > 6 decades, bottom > 5.5 decades; TRF > 6 decades; Lum > 7 decades |
| Sensitivity | FI (top) < 0.4 fmol fluorescein/well; FI (bottom) < 4 fmol fluorescein/well; TRF < 1 amol/well; Lum < 7 amol ATP/well; AlphaScreen < 100 amol phosphotyrosine/well |
| Photometric Accuracy | 0.003 Abs or ±2 % (200–399 nm, 0–2 Abs); ±1 % (400–1000 nm, 0–3 Abs) |
| Linearity | ±2 % (96-well 0–4 Abs @ 450 nm; 384-well 0–3 Abs) |
| Precision | SD < 0.001 Abs or CV < 0.5 % (450 nm, 0–3 Abs) |
| Wavelength Range | Abs 200–1000 nm; FI Exc. 200–822 nm / Em. 270–840 nm; TRF Exc. 334 nm / Em. 400–700 nm; Lum 360–670 nm; AlphaScreen Exc. 680 nm / Em. 400–660 nm |
| Incubator Temperature | Ambient + 4 °C to 45 °C |
| Plate Compatibility | 6 – 1536 wells (Absorbance: 6 – 384 wells); μDrop plates |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp and LED |
| Shaking | Orbital |
| Dimensions (L × W × H) | 58 × 53 × 51 cm (23 × 21 × 20 in.) |
| Weight | 59 kg (130 lb) |
| Interface | Thermo Scientific™ SkanIt™ Software |
| Read-out Range | 0 – 6 Abs |
| Product Line | Varioskan™ |
| Included Accessories | SkanIt PC Software (Research Edition), Nunc™ 96-Well Optical-Bottom Microplate (black, TC surface) |
Unique Advantages
- Dual dispensers enable simultaneous or staggered reagent injection for kinetic and enzyme-based assays.
- Comprehensive detection range supports fluorescence, luminescence, and absorbance assays within one platform.
- Automatic performance calibration ensures consistent accuracy and reliability.
- Validated assay compatibility for both HTRF™ and DLReady™ workflows.
- Upgradeable modular architecture protects your long-term investment.
Ordering Information
| Catalog Number | Measurement Technologies | Reading Options | Dispensers | Includes |
|---|---|---|---|---|
| VLBLATD2 | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence, AlphaScreen | Top and Bottom | 2 | SkanIt PC Software (Research Edition) |
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBLATD2) is an advanced dual-dispensing multimode system that offers unmatched sensitivity, speed, and versatility for complex assay formats. Its ability to combine absorbance, fluorescence, luminescence, TRF, and AlphaScreen detection makes it an indispensable tool for research and high-throughput laboratories seeking comprehensive multimode performance in a single platform.