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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence)
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Reading Options: Top and Bottom
No. of dispensers: 1
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TGD1) delivers exceptional versatility and performance for multimode microplate-based assays, integrating Absorbance, Fluorescence Intensity, Luminescence, and Time-Resolved Fluorescence (TRF) detection technologies in one modular system.
This configuration features a built-in Gas Control Module for precise CO₂ (0.1–15%) and O₂ (1–21%) regulation, combined with a single high-precision dispenser that enables real-time reagent addition and measurement—ideal for kinetic enzyme assays and luminescent reactions.
The Varioskan LUX combines automatic dynamic range selection, smart safety controls, and automated calibration, ensuring accuracy, reproducibility, and high throughput for both routine and advanced research workflows.
Key Features and Benefits
1. Four Integrated Measurement Technologies
- Detects Absorbance (UV-Vis, 200–1000 nm), Fluorescence Intensity, Luminescence, and Time-Resolved Fluorescence (TRF/TR-FRET).
- Top and bottom optics enhance flexibility for solution-based and cell-based assays.
- Dual monochromator optical system for precise excitation and emission wavelength control.
- Optional upgrade for AlphaScreen™ and AlphaLISA™ technologies.
2. Integrated Gas Control Module
- Built-in Gas Module enables regulation of CO₂ and O₂ levels, ideal for cell-based kinetic assays.
- Supports controlled incubation environments for live-cell analysis.
- Ensures physiological conditions for long-term monitoring of cellular responses.
3. Precision Dispensing System
- Single integrated dispenser for 2–5,000 μL reagent addition with 1 μL increments.
- Enables simultaneous dispensing and signal detection for enzyme kinetics and luminescence reactions.
- Ensures reproducibility and reduces human error in time-sensitive assays.
4. High Sensitivity and Performance
- Fluorescence Sensitivity: <0.4 fmol fluorescein/well (top read).
- TRF Sensitivity: <1 amol/well (white low-volume 384-well plate).
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
- Dynamic Range: Fluorescence >6 decades; Luminescence >7 decades; TRF >6 decades.
- Photometric Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0–3 Abs).
- Linearity: ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs).
5. Intelligent Automation and Workflow Optimization
- Automatic dynamic range selection for per-well signal optimization.
- Smart Safety Controls detect and prevent setup errors before assay initiation.
- Self-calibration and diagnostics maintain instrument accuracy and reliability.
- Multiple read modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Orbital shaking and temperature control (ambient +4°C to 45°C) for homogeneous reaction conditions.
6. Powerful SkanIt™ Software Integration
- Includes SkanIt™ PC Software (Research Edition) for comprehensive data acquisition and analysis.
- License-free installation for multiple users and workstations.
- Virtual Pipetting Tool for intuitive plate setup and assay mapping.
- Real-time kinetic and spectral visualization during measurements.
- Automatic data backup prevents data loss during interruptions.
- Built-in analytical tools: Blank subtraction, dose-response, curve fitting, kinetic modeling, and custom formulas.
- Export formats: *.xlsx, *.pdf, *.xml, *.txt; automatic emailing post-run.
7. Certified and Validated
- HTRF™ certified by Cisbio Bioassays.
- DLReady™ validated by Promega Corporation.
- Compliant with data integrity standards; 21 CFR Part 11 support available (Drug Discovery Edition).
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence |
| Reading Options | Top and Bottom |
| Dispensers | 1 (2–5,000 μL, 1 μL increments) |
| Gas Control Module | CO₂: 0.1–15%; O₂: 1–21% |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; TRF Excitation: 334 nm; TRF Emission: 400–700 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | FI: >6 decades; TRF: >6 decades; Luminescence: >7 decades |
| Sensitivity | FI: <0.4 fmol fluorescein/well; TRF: <1 amol/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 57 kg (125 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Compatibility | 6–1536 well plates; μDrop plates |
| Software Edition | SkanIt™ Research Edition |
Applications
- Kinetic Assays: Real-time enzyme reaction and inhibition monitoring with integrated dispensing.
- Cell-Based Assays: Reporter gene, cell viability, and cytotoxicity under controlled CO₂/O₂ environments.
- Fluorescence and TRF Assays: FRET/TR-FRET and HTRF-based applications.
- Luminescence Assays: ATP, luciferase, and bioluminescence detection.
- Nucleic Acid and Protein Quantification: DNA, RNA, and protein analysis via absorbance or fluorescence.
- Spectral Scanning: Excitation/emission profiling for assay optimization.
- Drug Discovery: High-throughput fluorescence and luminescence screening.
Unique Advantages
- Combines Absorbance, Fluorescence, Luminescence, and TRF detection technologies.
- Integrated Gas Control Module ensures stable cell-based assay environments.
- Built-in single dispenser allows reagent addition during live kinetic reads.
- Top and bottom reading optics deliver flexibility for diverse assay types.
- Automatic calibration and self-diagnostics ensure reproducible performance.
- HTRF™ and DLReady™ certifications guarantee validated compatibility.
- Upgradeable modular design allows expansion to AlphaScreen™ and TR-FRET technologies.
- SkanIt™ Software enables traceable, automated data acquisition and analysis.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TGD1) combines four advanced detection technologies with built-in gas control and integrated dispensing for unmatched flexibility in multimode assay workflows.
Engineered for precision, automation, and performance, it is ideal for kinetic enzymatic assays, fluorescence energy transfer applications, and live-cell analyses under controlled gas conditions.
Paired with SkanIt™ Software (Research Edition), this instrument provides real-time kinetic data, traceable analysis, and comprehensive assay control, empowering modern laboratories to achieve the highest standards in accuracy, sensitivity, and reproducibility.