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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence)
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Reading Options: Top and Bottom
No. of dispensers: 2
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TD2) combines four major detection technologies — Absorbance (UV-Vis), Fluorescence Intensity, Luminescence, and Time-Resolved Fluorescence (TRF) — in a single, modular, and high-performance platform.
Designed for advanced research laboratories, this configuration includes dual precision dispensers for real-time reagent addition and kinetic reaction monitoring, making it ideal for enzyme kinetics, luminescent ATP assays, and TR-FRET applications.
Engineered with smart automation, dynamic range optimization, and spectral scanning, the Varioskan LUX ensures high sensitivity, reproducibility, and flexibility for applications in cell biology, molecular research, biochemistry, and drug discovery.
Key Features and Benefits
1. Four Integrated Detection Technologies
- Absorbance (UV-Vis 200–1000 nm), Fluorescence Intensity (incl. FRET), Luminescence, and Time-Resolved Fluorescence (TRF/TR-FRET) in one instrument.
- Top and bottom optics for solution-based and cell-based assays.
- Dual monochromator system for precise control of excitation and emission wavelengths.
- Optional upgrades for AlphaScreen™ and AlphaLISA™ technologies.
2. Dual Dispensing System for Real-Time Kinetic Assays
- Two independent dispensers (2–5,000 μL, 1 μL increments) for simultaneous reagent addition and measurement.
- Enables immediate reaction initiation and real-time signal acquisition.
- Ideal for kinetic luminescence, enzyme catalysis, and TR-FRET assays requiring rapid mixing.
3. Superior Sensitivity and Dynamic Range
- Fluorescence Sensitivity: <0.4 fmol fluorescein/well (top read), <4 fmol fluorescein/well (bottom read).
- Time-Resolved Fluorescence Sensitivity: <1 amol/well (white low-volume 384-well plate).
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
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Dynamic Range:
- Fluorescence: >6 decades (top), >5.5 decades (bottom)
- TRF: >6 decades
- Luminescence: >7 decades
- Photometric Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm.
4. Smart Automation and Workflow Control
- Automatic dynamic range selection ensures optimal detection across wells.
- Smart Safety Controls identify setup errors before operation.
- Automated self-calibration and diagnostics guarantee long-term reliability.
- Five measurement modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Orbital shaking and temperature regulation (ambient +4°C to 45°C) maintain consistency for sensitive assays.
5. SkanIt™ Software Integration
- Includes SkanIt™ Research Edition for streamlined operation and analysis.
- License-free installation for multiple users.
- Virtual Pipetting Tool simplifies assay setup and plate configuration.
- Real-time kinetic and spectral data visualization during runs.
- Automatic data saving prevents loss during power interruptions.
- Built-in data analysis functions: Blank subtraction, curve fitting, kinetic modeling, and dose-response analysis.
- Export formats: *.xlsx, *.pdf, *.xml, *.txt, or automatic report emailing after completion.
6. Certified and Upgradeable
- HTRF™ certified by Cisbio Bioassays.
- DLReady™ validated by Promega Corporation.
- Modular design allows upgrades to AlphaScreen™ and AlphaLISA™ detection systems.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence |
| Reading Options | Top and Bottom |
| Dispensers | 2 (2–5,000 μL with 1 μL increments) |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; TRF Excitation: 334 nm; TRF Emission: 400–700 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence: Top >6 decades, Bottom >5.5 decades; TRF: >6 decades; Luminescence: >7 decades |
| Sensitivity | FI Top: <0.4 fmol fluorescein/well; FI Bottom: <4 fmol fluorescein/well; TRF: <1 amol/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 59 kg (130 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Compatibility | 6–1536 well plates; μDrop plates |
| Software Edition | SkanIt™ Research Edition |
Applications
- Kinetic and Enzymatic Assays: Real-time analysis of enzyme reactions and inhibition studies.
- Cell-Based Assays: Reporter gene, cell viability, and cytotoxicity assays using optical-bottom plates.
- Fluorescence and TRF Studies: FRET/TR-FRET and HTRF-based assays for biomolecular interactions.
- Luminescence Assays: ATP quantification, luciferase, and bioluminescence detection.
- Spectral Scanning: Excitation/emission optimization and spectral profiling.
- Drug Discovery and Screening: High-throughput assays using fluorescence and luminescence readouts.
Unique Advantages
- Combines Absorbance, Fluorescence, Luminescence, and Time-Resolved Fluorescence detection in one compact system.
- Dual dispensers enable simultaneous reagent addition for time-sensitive reactions.
- Dual top and bottom optics enhance assay versatility for multiple sample types.
- HTRF™ and DLReady™ certified for validated assay compatibility.
- Automatic calibration and dynamic range adjustment ensure reproducible data.
- Modular, upgradeable platform supports evolving research needs.
- Includes Nunc™ 96-Well Optical-Bottom Microplates for superior optical performance.
- SkanIt™ Software provides powerful data analysis, traceability, and automated reporting.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TD2) integrates four high-sensitivity detection technologies with dual dispensers, enabling real-time reagent addition and kinetic analysis for complex biological assays.
Equipped with automatic calibration, top/bottom optics, and advanced software integration, it offers unmatched flexibility, speed, and reproducibility across a wide range of applications including enzyme kinetics, TR-FRET, luminescence assays, and cell-based research.
With its modular design, HTRF™/DLReady™ validation, and included SkanIt™ Software and Nunc™ Optical-Bottom Plates, the Varioskan LUX is a complete, future-ready solution for today’s advanced research laboratories.