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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity)
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Reading Options: Top and bottom
No. of dispensers: 2
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader is an advanced, modular microplate reader designed for bioscience researchers who demand flexibility, accuracy, and reproducibility across a wide variety of assays.
Built on Thermo Fisher’s proven multimode detection platform, the Varioskan LUX integrates Absorbance, Fluorescence Intensity, and Luminescence detection technologies into a single, compact instrument.
With optional upgrades for AlphaScreen™, Time-Resolved Fluorescence (TRF), and TR-FRET/hTRF, this system delivers exceptional versatility for applications in molecular biology, cell-based assays, drug discovery, and enzyme kinetics.
The instrument includes a gas control module for CO₂ and O₂ regulation, making it ideal for live-cell assays, and features dual reagent dispensers for real-time kinetic monitoring.
Key Features and Benefits
1. Comprehensive Measurement Technologies
- Supports Absorbance (UV-Vis, 200–1000 nm), Fluorescence Intensity, and Luminescence modes.
- Optional expansion for AlphaScreen™, AlphaLISA™, and TRF applications.
- Combines filter and monochromator optics for superior sensitivity and flexibility.
- Top and bottom reading for diverse assay formats (6–1536 well plates).
2. Intelligent Automation and Workflow Efficiency
- Automatic Dynamic Range Selection optimizes detection for every well.
- Simultaneous dispensing and measurement enables kinetic signal monitoring at reaction initiation.
- Dual dispensers allow precise reagent addition for homogeneous and kinetic assays.
- Gas Control Module maintains CO₂ (0.1–15%) and O₂ (1–21%) concentrations for cell-based assays.
- Smart Safety Controls detect and prevent user or setup errors automatically.
3. Reliable, High-Performance Results
- Precision photomultiplier tube (PMT) detector with wide dynamic range.
- Accuracy: ±2% (200–399 nm) and ±1% (400–1000 nm).
- Sensitivity: Fluorescence <0.4 fmol fluorescein/well; Luminescence <7 amol ATP/well.
- Linearity: ±2% for 96- and 384-well plates.
- Carryover reduction: Non-contact dispensing and orbital shaking ensure uniform mixing and accuracy.
4. Powerful SkanIt™ Software Integration
- License-free installation for multiple users.
- Virtual Pipetting Tool simplifies sample-to-plate layout.
- Real-time spectra and kinetic curve visualization.
- Automatic data saving prevents loss during interruptions.
- Built-in data analysis: Blank subtraction, curve fitting, dose-response, and QC metrics.
- Flexible exports: *.xlsx, *.pdf, *.xml, and *.txt formats; auto-email function post-run.
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Two editions available:
- Research Edition: Standard for life science research.
- Drug Discovery Edition: Includes 21 CFR Part 11 compliance features.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence |
| Reading Options | Top and Bottom |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm, Emission: 270–840 nm; Luminescence: 360–670 nm |
| Dispensers | 2 |
| Dispense Volume | 2–5,000 μL (1 μL increments) |
| Measurement Speed | 15 s (96-well); 45 s (384-well); 135 s (1536-well) |
| Dynamic Range | Fluorescence: >6 decades; Luminescence: >7 decades |
| Sensitivity | Fluorescence: <0.4 fmol fluorescein/well; Luminescence: <7 amol ATP/well |
| Precision | SD <0.001 Abs; CV <0.5% at 450 nm |
| Linearity | ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs) |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp |
| Gas Control | CO₂: 0.1–15%; O₂: 1–21% |
| Temperature Control | Ambient +4°C to 45°C |
| Plate Types | 6–1536 well plates; μDrop plates |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 57 kg (125 lb) |
| Interface | PC Software (SkanIt Software) |
| Shaking | Orbital |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
Applications
- Molecular Biology: Nucleic acid and protein quantification (A260/A280).
- Cell-Based Assays: Reporter gene assays, cytotoxicity, cell proliferation, and viability.
- Enzyme Kinetics: Kinetic assays for enzyme activation and inhibition.
- Drug Discovery: Quantitative high-throughput screening.
- Immunoassays: ELISA, AlphaLISA™, TR-FRET, and luminescent assays.
- Luminescence Studies: ATP detection, luciferase, and glow assays.
- Environmental and Biochemical Research: Spectral scanning and compound screening.
Unique Advantages
- Combines Absorbance, Fluorescence, and Luminescence detection in one modular platform.
- Integrated Gas Control Module for live-cell and physiological assays.
- Dual dispenser system for kinetic and homogeneous assays.
- Automatic dynamic range selection and smart calibration.
- Comprehensive HTRF™ and DLReady™ validation for assay compatibility.
- Paired with intuitive SkanIt™ Software for complete data control and reporting.
- Modular and upgradable design to meet evolving laboratory needs.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00GD2) offers unmatched versatility, combining Absorbance, Fluorescence, and Luminescence detection in a modular platform equipped with gas control and dual dispensers.
Its advanced automation, intelligent safety controls, and powerful SkanIt™ Software make it a trusted choice for research laboratories engaged in molecular biology, drug discovery, and biochemical assay development.
A validated and upgradable solution designed for consistent performance, flexibility, and ease of use in high-demand research environments.