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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence)
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Reading Options: Top and bottom
No. of dispensers: 2
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00D2) is a high-performance, modular system offering absorbance (UV-Vis), fluorescence intensity, and luminescence detection with dual precision dispensers for kinetic and homogeneous assay workflows.
Built to meet the demands of life science, pharmaceutical, and academic laboratories, this system enables real-time signal monitoring during reagent addition, significantly improving reproducibility for time-critical experiments such as enzyme kinetics, ATP quantification, and luminescence assays.
With advanced features such as automatic dynamic range selection, smart safety controls, and automated calibration, the Varioskan LUX ensures reliable, error-free operation for both routine and advanced multimode applications.
Key Features and Benefits
1. Advanced Multimode Detection
- Combines Absorbance (UV-Vis 200–1000 nm), Fluorescence Intensity, and Luminescence technologies.
- Top and bottom reading optics for optimal detection in solution-based and adherent cell-based assays.
- Dual monochromator system provides precise wavelength selection for excitation and emission.
- Spectral scanning for full-wavelength optimization and spectral assay analysis.
- Compatible with 6–1536 well plates and μDrop plates for low-volume applications.
2. Dual Dispensing System for Kinetic Precision
- Two built-in dispensers (2–5,000 μL, 1 μL increments) enable simultaneous reagent addition and measurement.
- Perfect for rapid enzymatic reactions, luminescence-based kinetic assays, and homogeneous mixing.
- Reduces operator variability and enhances assay reproducibility.
3. High Sensitivity and Dynamic Range
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Fluorescence Sensitivity:
- Top: <0.4 fmol fluorescein/well.
- Bottom: <4 fmol fluorescein/well.
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
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Dynamic Range:
- Fluorescence: >6 decades (top), >5.5 decades (bottom).
- Luminescence: >7 decades.
- Photometric Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0–3 Abs).
4. Intelligent Automation and Workflow Efficiency
- Automatic dynamic range selection per well for optimal signal intensity.
- Smart Safety Controls detect potential setup errors before execution.
- Self-calibration and diagnostics ensure consistent accuracy and reliability.
- Five measurement modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Orbital shaking and temperature control (ambient +4°C to 45°C) for stable assay conditions.
5. Powerful SkanIt™ Software Integration
- Includes Thermo Scientific™ SkanIt™ Software (Research Edition).
- License-free multi-user access allows flexible installation across devices.
- Virtual Pipetting Tool for intuitive sample mapping and setup.
- Real-time kinetic and spectral data visualization during runs.
- Automatic data saving prevents loss during power interruptions.
- Built-in data analysis tools: Blank subtraction, curve fitting, kinetic modeling, dose-response analysis, and QC checks.
- Export options: *.xlsx, *.pdf, *.xml, *.txt; automatic report emailing available.
6. Certified and Upgradeable
- HTRF™ certified by Cisbio Bioassays.
- DLReady™ validated by Promega Corporation.
- Modular design supports future expansion to AlphaScreen™, TRF, and TR-FRET/hTRF detection technologies.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence |
| Reading Options | Top and Bottom |
| Dispensers | 2 (2–5,000 μL, 1 μL increments) |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence: Top >6 decades, Bottom >5.5 decades; Luminescence >7 decades |
| Sensitivity | FI Top: <0.4 fmol fluorescein/well; FI Bottom: <4 fmol fluorescein/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well 0–4 Abs; 384-well 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 1 flash, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Interface | PC Software (SkanIt™ Software) |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 58 kg (128 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Formats | 6–1536 well plates; μDrop plates |
| Software Edition | Research Edition |
Applications
- Kinetic and Enzyme Assays: Real-time kinetic measurement of enzyme catalysis, inhibition, and substrate turnover.
- Cell-Based Assays: Reporter gene, viability, proliferation, and cytotoxicity analysis.
- Luminescent Assays: ATP quantification, luciferase expression, and cell signaling detection.
- Fluorescence Studies: FRET, GFP, and fluorescent probe-based assays.
- Protein and Nucleic Acid Quantification: Quantitative DNA/RNA and protein concentration measurement.
- Drug Screening: Dual-dispensing functionality enables complex kinetic compound testing.
- Spectral Scanning: Wavelength optimization for assay development and validation.
Unique Advantages
- Combines Absorbance, Fluorescence, and Luminescence detection in one modular instrument.
- Dual dispensers enable real-time reagent addition and kinetic reaction monitoring.
- Dual top and bottom reading optics maximize assay flexibility.
- Automated calibration, dynamic range selection, and diagnostics guarantee reproducibility.
- HTRF™ and DLReady™ certifications ensure validated performance across applications.
- Modular, upgradable design supports future expansion into advanced detection technologies.
- SkanIt™ Software offers full data traceability, real-time visualization, and analysis automation.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00D2) offers exceptional flexibility and sensitivity across absorbance, fluorescence, and luminescence detection modes, now enhanced with dual reagent dispensers for real-time kinetic assays.
With automatic calibration, dynamic range optimization, and intelligent software integration, it ensures accurate, reproducible, and traceable results for research laboratories worldwide.
An ideal choice for molecular biology, drug discovery, and biochemistry laboratories, the Varioskan LUX combines automation, precision, and versatility in one powerful platform.