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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence)
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Reading Options: Top
No. of dispensers: None
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VL0L00D0) offers a flexible, accurate, and efficient platform for multimode microplate-based assays.
It integrates absorbance (UV-Vis), fluorescence intensity, and luminescence measurement technologies within a compact and modular system designed to meet the needs of modern bioscience researchers.
Engineered for assay flexibility, performance reliability, and ease of use, this system supports a wide range of applications including cell-based assays, enzyme kinetics, DNA/RNA quantification, and luminescent reporter analysis.
The Varioskan LUX features automatic dynamic range selection, smart safety controls, and powerful SkanIt™ Software—ensuring consistent, high-quality data with minimal setup and user intervention.
Key Features and Benefits
1. Multimode Detection Capabilities
- Combines Absorbance (UV-Vis 200–1000 nm), Fluorescence Intensity, and Luminescence detection.
- Optimized top reading optics ideal for solution-based and homogeneous assays.
- Dual monochromator design enables flexible wavelength selection for excitation and emission.
- Compatible with 6–1536 well plates and μDrop plates for small-volume samples.
- Spectral scanning for assay optimization and peak wavelength identification.
2. Enhanced Sensitivity and Dynamic Range
- Fluorescence Sensitivity: <0.4 fmol fluorescein/well (top read).
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
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Dynamic Range:
- Fluorescence: >6 decades.
- Luminescence: >7 decades.
- Photometric Accuracy: ±2% (200–399 nm) and ±1% (400–1000 nm).
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0–3 Abs).
- Linearity: ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs at 450 nm).
3. Reliable and Automated Operation
- Automatic dynamic range selection ensures optimal signal intensity per well.
- Smart safety controls detect errors before they impact results.
- Automated self-calibration and instrument diagnostics maintain precision over time.
- Multiple measurement modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Orbital shaking and temperature regulation (ambient +4°C to 45°C) for consistent assay conditions.
4. SkanIt™ Software for Intuitive Data Control
- Includes Thermo Scientific™ SkanIt™ PC Software (Research Edition).
- License-free multi-user installation for flexible access.
- Virtual Pipetting Tool simplifies sample layout and experimental setup.
- Real-time spectra and kinetic curve display.
- Automatic data saving prevents loss during power interruptions.
- Built-in calculations: Blank subtraction, standard curve, dose-response, kinetic, spectral, and custom formula analysis.
- Flexible export formats: *.xlsx, *.pdf, *.xml, *.txt; automated email report option.
5. Certified and Upgradeable
- HTRF™ certified by Cisbio Bioassays for homogenous TR-FRET applications.
- DLReady™ validated by Promega Corporation for luminescence assays.
- Modular design allows future upgrades to include AlphaScreen™, TR-FRET, and TRF technologies.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence |
| Reading Options | Top |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence: >6 decades; Luminescence: >7 decades |
| Sensitivity | <0.4 fmol fluorescein/well (fluorescence); <7 amol ATP/well (luminescence) |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Interface | PC Software (SkanIt™ Software) |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 56 kg (123 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Formats | 6–1536 well plates (Absorbance up to 384 wells), μDrop plates |
| Software Edition | Research Edition |
Applications
- Molecular Biology: DNA, RNA, and protein quantification using absorbance and fluorescence.
- Enzyme Kinetics: Real-time enzyme activity and inhibition monitoring.
- Cell-Based Assays: Reporter gene, viability, and cytotoxicity studies.
- Luminescent Assays: ATP detection, luciferase gene expression, and bioluminescence.
- Fluorescence-Based Studies: FRET, GFP, and probe-based analysis.
- Spectral Scanning: Excitation/emission optimization for assay development.
- Drug Discovery: High-throughput fluorescence and luminescence screening.
Unique Advantages
- Integrates Absorbance, Fluorescence Intensity, and Luminescence in one platform.
- Optimized top reading optics for microplate and solution-based assays.
- Automatic calibration and dynamic range control enhance reproducibility.
- HTRF™ and DLReady™ certifications ensure validated performance.
- Modular design for future technology upgrades.
- SkanIt™ Software provides powerful, intuitive control and comprehensive data management.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VL0L00D0) delivers high-performance multimode detection across absorbance, fluorescence, and luminescence technologies.
Engineered for reliability, sensitivity, and modular flexibility, it offers automated calibration, smart error prevention, and seamless data control through SkanIt™ Software.
Ideal for research laboratories, biotechnology centers, and pharmaceutical facilities, the Varioskan LUX simplifies multimode assay workflows while maintaining accuracy and reproducibility for every application.