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Specifications:
| Application | qPCR, Reverse Transcription | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | SolisBiodyne | ||
| Product Grade | Molecular Biology | ||
The SolisFAST® Lyo-Ready qPCR Kit with UNG is a fast, sensitive, and glycerol-free qPCR solution optimized for lyophilization into beads or cakes. The kit combines a 5× SolisFAST® Lyo-compatible qPCR Mix with UNG and a 4× proprietary excipient mix, ensuring reliable performance both before and after freeze-drying.
By incorporating dUTPs and Salini UNG® Uracil-N-Glycosylase, the kit prevents carryover contamination, delivering accurate and reproducible results. Its compatibility with fast cycling protocols enables results in as little as 30 minutes, making it ideal for high-throughput and diagnostic research applications.
Key Features & Benefits
- Lyophilization Ready – Glycerol-free composition with excipient mix for stable lyo-beads or lyo-cakes.
- Contamination Control – Contains dUTPs and UNG to eliminate carryover contamination.
- Fast Cycling – Generates reliable results in as little as 30 minutes.
- Sensitive & Specific – Supports detection and quantification of DNA and cDNA with high accuracy.
- Multiplexing – Detect up to five targets simultaneously.
- Long-Term Stability – Maintains performance after lyophilization, even under stress conditions (e.g., +37°C).
- Clean Data – No false positives, consistent Cq values across liquid and lyophilized reagents.
Kit Components
- 5× SolisFAST® Lyo-compatible qPCR Mix with UNG (polymerase, dUTPs, MgCl₂, buffer, Salini UNG®)
- 4× SolisFAST® Lyo Excipient Mix (proprietary stabilizers and cryoprotectants for lyophilization)
Applications
- DNA and cDNA detection and quantification
- Lyophilization workflows – compatible with bead or cake formats
- DNA/LNA hydrolysis probe-based assays (e.g., TaqMan®)
- Singleplex and multiplex detection (up to 5 targets)
- Microbial and viral detection assays
- Genotyping workflows
- Field-deployable and portable qPCR assay development
Product Specifications
| Attribute | Detail |
|---|---|
| Format | Glycerol-free, lyo-ready qPCR kit |
| Enzyme | Hot-start polymerase (activation 30 sec–3 min at 95°C) |
| Detection Type | Probe-based (hydrolysis probes) |
| Cycling Speed | Fast and ultra-fast protocols |
| Reference Dye | None (ROX optional for ROX-dependent platforms) |
| Multiplexing Capacity | Up to 5-plex |
| Contamination Prevention | dUTP + Salini UNG® |
| Sample Types | DNA, cDNA |
| Storage | –20°C (routine), 2 weeks stable at room temperature |
| Shipping | Ice packs |
Ordering Information
| Catalog Number | Size | Reaction Capacity |
|---|---|---|
| 28-52-0000S | 0.4 mL + 0.5 mL | 100 rxn |
| 28-52-00250 | — | 250 rxn |
| 28-52-00250-5 | 5 × 250 rxn | 1250 rxn |
| 28-52-05000 | — | 5000 rxn |
The SolisFAST® Lyo-Ready qPCR Kit with UNG is a next-generation, glycerol-free solution designed for fast, contamination-free, and multiplex-capable qPCR. With its dual-component system (lyo-compatible mix + excipient mix), it ensures excellent stability and reproducibility both in liquid form and after lyophilization, making it an ideal choice for diagnostic assay development, portable workflows, and high-throughput molecular research.
- Pack Size: 250 reactions 100 rxn, free sample 5000 Reactions 1250 Reactions