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Specifications:
| Application | Isothermal Amplification | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | SolisBiodyne | ||
| Product Grade | Molecular Biology | ||
The SoliSD™ Lyo-compatible RT-LAMP Kit is a glycerol-free, lyophilization-compatible isothermal amplification system designed for rapid and reliable RNA and DNA detection. Built on the powerful SoliSD™ Bsm DNA Polymerase with strong strand displacement activity and the patented Stability TAG™ technology, this kit provides room temperature stability for ≥1 month and robust performance across diverse assay formats.
The kit features a unique 300× RT-Mix, combining Solis BioDyne reverse transcriptases with an RNase inhibitor, ensuring efficient reverse transcription and superior performance in RT-LAMP assays. Optimized to run at 55–60°C (optimum 59°C), this kit enables highly sensitive detection with no NTC (non-template control) signal and results in as little as 4 minutes.
Key Features & Benefits
- Lyophilization Compatible – Glycerol-free enzyme formulation for freeze- or air-drying workflows.
- Room Temperature Stability – Stable for ≥1 month at 37°C, simplifying shipping and storage.
- Strong Strand Displacement – Powered by SoliSD™ Bsm DNA Polymerase for efficient amplification.
- Integrated RT-Mix – Proprietary blend of reverse transcriptases and RNase inhibitor for reliable RNA detection.
- No Background Amplification – Clean results without NTC signals.
- Fast Time-to-Result – Detect amplification products in as little as 4 minutes.
- Optimized RT-LAMP Performance – Operates effectively at 55–60°C (optimum 59°C).
Kit Components (5-vial format)
- SoliSD™ Glycerol-Free Bsm DNA Polymerase (40 U/µL) – 1 vial
- 300× RT Mix (FIREScript®, SOLIScript®, RiboGrip® blend + RNase inhibitor) – 1 vial
- 10× RT-LAMP Reaction Buffer – 1 vial
- 100 mM MgSO₄ – 1 vial
- 10× GC-rich Enhancer – 1 vial
Applications
- RT-LAMP workflows for RNA/DNA detection
- Rapid diagnostic assay development
- Portable and lyophilized testing systems
- GC-rich template amplification
- Field and point-of-care molecular diagnostics
Product Specifications
| Attribute | Detail |
|---|---|
| Format | 5-vial kit, glycerol-free |
| Polymerase Source | Large fragment Bsm DNA polymerase (Bacillus smithii) |
| Enzyme Activity | Strong strand displacement |
| Exonuclease Activity | None (no 5′→3′ or 3′→5′) |
| Temperature Range | 55–60°C (optimum 59°C) |
| Detection Time | As little as 4 minutes |
| Stability | ≥1 month at 37°C |
| Storage | –20°C (routine) |
| Shipping | Ice packs |
Ordering Information
| Catalog Number | Size | Reaction Capacity |
|---|---|---|
| 32-23-0000S | Small | 100 rxn |
| 32-23-00250 | Medium | 250 rxn |
| 32-23-01000 | Large | 1000 rxn |
| 32-23-05000 | Bulk | 5000 rxn |
The SoliSD™ Lyo-compatible RT-LAMP Kit delivers a powerful, glycerol-free isothermal amplification solution tailored for RNA detection and diagnostic assay development. With Stability TAG™ technology, an integrated reverse transcriptase mix, and lyophilization compatibility, it is ideal for laboratories and developers building sensitive, portable, and field-deployable molecular testing platforms.
- Pack Size: 250 reactions 1000 Reactions 100 rxn, free sample 5000 Reactions