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Invitrogen™ PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside), 5mg
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Specifications:
| Application | Fluorescence-Based Assay | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Live-cell, fluorescent glucocerebrosidase (GCase) activity probe for high-content and single-cell analysis
Invitrogen™ PFB-FDGlu is a cell-permeable, fluorescein-based substrate designed for live-cell imaging and flow cytometric analysis of lysosomal glucocerebrosidase (GCase) activity. GCase is a critical lysosomal hydrolase in glycolipid metabolism, and its deficiency is implicated in diseases such as Gaucher’s disease and Parkinson’s disease.
PFB-FDGlu contains a pentafluorobenzoylamino-modified fluorescein moiety linked to two β-D-glucopyranoside groups. Once loaded into cells, the substrate localizes to endosomal and lysosomal compartments. Upon cleavage by active GCase, it releases a bright green fluorescent dye (PFB-F; excitation/emission ~492/516 nm) that remains cell-associated, enabling specific, high-sensitivity detection of GCase activity.
Key Features
- High Specificity – Designed for GCase detection in lysosomes with minimal off-target hydrolysis
- Live-Cell Compatible – Enables functional enzyme activity assays in viable cells
- Bright, Retained Signal – Cleaved product is cell-associated for prolonged detection
- Multiplex-Friendly – Compatible with viability stains and antibody markers for phenotyping and cell sorting
- Versatile Readouts – Suitable for flow cytometry, fluorescence microscopy, confocal imaging, and high-content screening
Applications
- GCase activity quantification in live mammalian cells
- Disease research in Gaucher’s and Parkinson’s disease models
- High-content imaging for lysosomal function assays
- Flow cytometry-based single-cell GCase activity profiling
- Multiparametric analysis combining enzyme activity with cell viability or phenotypic markers
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | P11947 (also known as P-11947) |
| Quantity | 5 mg |
| Chemical Name | 5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside |
| Target Enzyme | β-Glucosidase (GCase) |
| Excitation/Emission | ~492/526 nm |
| Form | Powder |
| Cell Permeability | Yes |
| Detection Method | Fluorescence |
| Compatible Equipment | Flow cytometer, high-content imaging, fluorescent/confocal microscope |
| Storage Conditions | –5°C to –30°C |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
The PFB-FDGlu substrate offers researchers a precise, sensitive, and cell-permeant tool for monitoring lysosomal GCase activity in real time. Its design makes it ideal for disease modeling, functional lysosomal assays, and multiparameter single-cell analysis, bridging cell biology, enzymology, and translational research.