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Invitrogen™ Bolt™ Welcome Pack with iBlot™ 3 System
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Specifications:
| Application | Protein Electrophoresis & Western Blotting | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Bolt Welcome Pack with iBlot 3 System provides an all-inclusive solution for efficient protein electrophoresis and western blotting workflows. This kit includes everything needed for gel electrophoresis, protein separation, and fast, reliable western blot transfers.
Key Components and Features
1. iBlot™ 3 Western Blot Transfer Device
- Dry Transfer Technology
- Eliminates the need for liquid buffers, enabling faster transfer compared to traditional wet methods.
- Transfers are completed in as little as three minutes with consistent and reliable results.
- Membrane Compatibility
- Works seamlessly with both nitrocellulose and PVDF membranes.
- Sustainability
- Reduced waste and environmentally conscious design.
2. Bolt™ Bis-Tris Plus Gels
- Precast Gels for Optimal Protein Separation
- Designed for broad-range protein separation under denaturing conditions.
- Neutral pH minimizes protein modifications, ensuring more accurate results.
- Unique WedgeWell Design
- Easier sample loading with up to 2x more sample volume capacity than standard gels.
- Separation Range
- Ideal for resolving proteins in the range of 3.5 to 260 kDa.
3. Mini Gel Tank
- Compatible with Various Gel Formats
- Accommodates Novex, NuPAGE, and Bolt Bis-Tris Plus gels.
- Allows for simultaneous running of up to two gels per run.
- Efficient Operation
- Optimized for running gels in approximately 20 minutes with MES running buffer.
4. Bolt Buffers and Reagents
- MES Running Buffer (20X)
- For optimal separation of medium-to-low molecular weight proteins.
- LDS Sample Buffer (4X)
- Ensures proper protein denaturation and sample preparation.
- Sample Reducing Agent (10X)
- Facilitates disulfide bond reduction for accurate protein analysis.
5. PageRuler™ Prestained Protein Ladder
- Molecular Weight Marker
- Covers a range of 10 to 250 kDa for precise molecular weight determination.
Specifications
| Feature | Details |
|---|---|
| For Use With (Application) | Western Blot, Protein Electrophoresis |
| Gel Size | Mini |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Gel Percentage | 4 to 12% |
| Gel Type | Bis-Tris Plus |
| Separation Range | 3.5 to 260 kDa |
| Thickness | 1.0 mm |
| No. of Wells | 10-well |
| Shipping Condition | Room Temperature or Wet Ice |
| Green Features | Less waste, Sustainable packaging |
Applications
- Protein Separation
- Ideal for resolving proteins across a broad range of molecular weights.
- Western Blotting
- Efficient protein transfer for nitrocellulose and PVDF membranes.
- Protein Analysis
- Suitable for research involving protein characterization, quantification, and interaction studies.
Contents of the Welcome Pack
- iBlot™ 3 Western Blot Transfer Device
- iBlot™ 3 Transfer Stacks, Midi, Nitrocellulose (1 box)
- Mini Gel Tank
- Bolt™ 4-12% Bis-Tris Plus Gels, 10-Well (1 box)
- Bolt™ MES Running Buffer (20X, 500 mL)
- Bolt™ LDS Sample Buffer (4X, 10 mL)
- Bolt™ Sample Reducing Agent (10X, 10 mL)
- PageRuler™ Plus Prestained Protein Ladder (10 to 250 kDa, 2 x 250 μL)
Benefits
- Complete Workflow Solution
- From protein separation to western blotting, all necessary components are included.
- Time-Saving
- Rapid gel runs and ultra-fast transfer times streamline workflows.
- Environmentally Friendly
- Less waste and sustainable packaging.
- User-Friendly Design
- Easy-to-use systems with high compatibility and reproducible performance.
Why Choose the Bolt™ Welcome Pack with iBlot™ 3 System?
This system offers a comprehensive, time-efficient, and reliable solution for laboratories conducting protein electrophoresis and western blotting. It ensures superior protein separation and transfer quality, making it an ideal choice for researchers seeking convenience and performance in one package.