Invitrogen™ NativePAGE™ Bis-Tris Mini Protein Gels, 4 to 16%, 1.0 mm

The Invitrogen™ NativePAGE™ Bis-Tris Mini Protein Gels are specialized precast polyacrylamide gels designed for the high-resolution analysis of native proteins and protein complexes. These gels are ideal for molecular mass estimation, purity assessment, and the study of protein conformations and interactions, particularly in membrane-protein complexes. They leverage the innovative blue native polyacrylamide gel electrophoresis (BN PAGE) technique for superior resolution and stability.

Key Features:

1. Wide Molecular Weight Range:
   • Resolves proteins and complexes from 15 kDa to 10,000 kDa.
2. Neutral pH Environment:
   • Operates at near-neutral pH, preserving native states of protein complexes and providing gel matrix stability.
3. Detergent Compatibility:
   • Compatible with Coomassie G-250, enabling analysis of membrane proteins without denaturation.
4. Superior Resolution:
   • Provides sharper bands compared to traditional Tris-glycine systems, regardless of protein isoelectric points (pI).
5. Ease of Use:
   • Precast gel format simplifies setup, reduces preparation time, and ensures consistent results.

Applications:

• Native protein electrophoresis.
• Analysis of protein-protein interactions and complexes.
• Membrane protein studies in native conformations.
• Molecular mass determination and purity evaluation.

How It Works:

• Instead of SDS, BN PAGE uses Coomassie G-250 as a charge-shifting molecule, imparting a net negative charge to proteins while maintaining their native structure.
• Proteins migrate based on size and charge under a near-neutral pH, ensuring stability during electrophoresis.

Technical Specifications:

• Gel Composition: Bis-Tris polyacrylamide.
• Gel Percentage: 4–16%.
• Thickness: 1.0 mm.
• Sample Loading Volume: Up to 15 μL.
• Separation Range: 15–10,000 kDa.
• Well Formats: Available in 10-well and 15-well configurations.
• Shelf Life: 8 months when stored at 2–8°C (do not freeze).
• Shipping Condition: Wet ice.

Recommended Use and Accessories:

• Electrophoresis Tanks: Compatible with Mini Gel Tank and XCell SureLock Mini-Cell systems.
• Blotting Systems:
  • NuPAGE Transfer Buffers for wet transfer to PVDF membranes.
  • Invitrogen Power Blotter or iBlot 2 Gel Transfer Device for rapid dry or semi-dry transfer.
• Membranes: Use PVDF membranes; nitrocellulose is not recommended due to Coomassie G-250 dye binding.

Storage and Stability:

• Store at 2°C to 8°C. Avoid freezing to maintain gel integrity.

Packaging:

• Each box contains 10 gels.

Use Cases:

1. Molecular Mass Estimation: High-resolution separation for determining the size of native protein complexes.
2. Protein Purity Assessment: Analyze purity and detect contaminant proteins.
3. Membrane Protein Research: Study structural and functional aspects of membrane proteins.
4. Protein-Protein Interaction Studies: Investigate complex formations under native conditions.

Advantages Over Traditional Systems:

• Enhanced protein stability due to neutral pH conditions.
• Compatibility with detergent-containing samples.
• Resolves a broad range of protein sizes with sharper bands.

For Research Use Only: Not intended for diagnostic applications.

For additional information, visit the Invitrogen website or refer to product documentation.