Liofilchem™ MTS™ Minocycline MN 0.016-256, MIC Test strips

The Liofilchem™ MTS™ Minocycline (MN) MIC Test Strips are a precise diagnostic tool for determining the minimum inhibitory concentration (MIC) of minocycline against bacterial pathogens. These gradient-based strips are essential in clinical microbiology, research, and pharmaceutical settings for assessing bacterial susceptibility to minocycline, a broad-spectrum tetracycline antibiotic.

Key Features:

1. Wide MIC Range:
   • Detects MIC values from 0.016 to 256 µg/mL, accommodating a broad range of bacterial susceptibilities.
2. Advanced Gradient Technology:
   • Creates a continuous concentration gradient of minocycline on the strip for precise MIC determination.
3. High-Quality Manufacturing:
   • Complies with CLSI (Clinical and Laboratory Standards Institute) and EUCAST (European Committee on Antimicrobial Susceptibility Testing) guidelines for accuracy and reliability.
4. Ready-to-Use:
   • Pre-calibrated and pre-sterilized strips simplify testing and reduce preparation time.
5. Broad Spectrum of Activity:
   • Effective against gram-positive and gram-negative bacteria, including multidrug-resistant strains.
6. Cost-Effective and Efficient:
   • Eliminates the need for serial dilutions, reducing labor and reagent usage.

Applications:

1. Clinical Diagnostics:
   • Determines bacterial susceptibility to minocycline, guiding effective antimicrobial therapy.
2. Research and Development:
   • Supports studies on resistance mechanisms, efficacy of minocycline, and new treatment strategies.
3. Pharmaceutical Quality Control:
   • Ensures the efficacy of minocycline formulations during drug development and production.
4. Antimicrobial Resistance Surveillance:
   • Monitors resistance trends in pathogens for public health purposes.

Use Cases:

1. Respiratory Infections:
   • Assesses MIC for pathogens such as Haemophilus influenzae and Streptococcus pneumoniae.
2. Skin and Soft Tissue Infections:
   • Evaluates susceptibility of Staphylococcus aureus (including MRSA) and Streptococcus pyogenes.
3. Urinary Tract Infections (UTIs):
   • Determines minocycline MIC for Escherichia coli and Enterococcus faecalis.
4. Multidrug-Resistant Infections:
   • Tests efficacy against resistant strains like Acinetobacter baumannii and Klebsiella pneumoniae.
5. Bloodstream Infections:
   • Identifies MIC for pathogens causing sepsis, such as Enterobacter cloacae and Pseudomonas aeruginosa.

Specifications:

Testing Procedure:

1. Preparation:
   • Inoculate a Mueller-Hinton agar plate with a standardized bacterial suspension (e.g., 0.5 McFarland standard).
2. Strip Application:
   • Using sterile forceps or an applicator, place the MTS™ Minocycline strip on the agar surface.
3. Incubation:
   • Incubate the plate at 35°C for 16–20 hours in an aerobic atmosphere.
4. MIC Reading:
   • Observe the inhibition ellipse intersecting the strip.
   • Read the MIC value where the ellipse touches the strip scale.

Advantages:

1. Precision and Accuracy:
   • Gradient technology enables highly accurate MIC determination, ensuring reliable results.
2. Ease of Use:
   • Ready-to-use strips minimize preparation time and handling errors.
3. Broad Applicability:
   • Effective against a wide spectrum of bacterial species, including multidrug-resistant strains.
4. Validated Performance:
   • Regular quality control ensures compliance with international standards.

Pathogens Typically Tested:

• Staphylococcus aureus (including MRSA)
• Escherichia coli
• Haemophilus influenzae
• Acinetobacter baumannii
• Klebsiella pneumoniae
• Pseudomonas aeruginosa
• Enterobacter cloacae
• Streptococcus pneumoniae

Quality Control:

• Control Strains:
  • Escherichia coli ATCC® 25922 (susceptible).
  • Staphylococcus aureus ATCC® 29213 (susceptible).
  • Pseudomonas aeruginosa ATCC® 27853 (negative control, resistant).
• Routine quality control ensures consistent and reproducible results.

Storage and Handling:

• Store at 2°C to 8°C in a dry, dark environment.
• Avoid exposure to moisture or prolonged room temperature storage.
• Use strips before the labeled expiration date.

Product Details:

Use in Clinical and Research Settings:

• Infection Management:
  • Guides antimicrobial therapy by providing precise MIC values for resistant and sensitive strains.
• Public Health:
  • Monitors emerging resistance patterns in clinical and environmental isolates.
• Pharmaceutical Development:
  • Supports new drug formulations by assessing minocycline activity.

The Liofilchem™ MTS™ Minocycline MIC Test Strips are a reliable and precise tool for determining bacterial susceptibility to minocycline. Their advanced gradient technology, broad-spectrum application, and ease of use make them an indispensable resource in clinical diagnostics, antimicrobial resistance surveillance, and microbiological research.