Invitrogen™ SuperScript™ IV VILO™ Master Mix

Invitrogen™ SuperScript™ IV VILO™ Master Mix is a high-performance reverse transcription master mix designed for rapid, sensitive, and reproducible first-strand cDNA synthesis for RT-qPCR applications. Built on the advanced SuperScript™ IV Reverse Transcriptase platform, this master mix delivers superior cDNA yield, improved sensitivity, and enhanced inhibitor tolerance, even when working with low-input or partially degraded RNA samples.

The kit is available with or without ezDNase, which enables fast and efficient removal of contaminating genomic DNA in as little as 2 minutes, significantly simplifying workflows and improving RT-qPCR accuracy. With fast reaction times of just 10 minutes, reduced pipetting steps, and optimized buffer chemistry, SuperScript IV VILO Master Mix ensures consistent and reliable gene expression analysis across a wide range of sample types.

This master mix is ideal for applications requiring high precision, including gene expression profiling, biomarker validation, and next-generation sequencing workflows.

Key Features and Benefits

Ultra-Fast cDNA Synthesis

• Complete reverse transcription in as little as 10 minutes
• Accelerates RT-qPCR workflows without compromising performance

Superior Sensitivity and Yield

• Produces higher cDNA yields than conventional RT systems
• Reduces Ct values by more than two cycles compared to other reverse transcriptases
• Supports detection of low-abundance transcripts

Optional Integrated Genomic DNA Removal

• ezDNase removes genomic DNA contamination in just 2 minutes
• Minimizes sample loss and improves data accuracy
• Eliminates need for separate DNase treatment step

Highly Thermostable SuperScript IV Reverse Transcriptase

• Improved thermostability and processivity
• Reduced RNase H activity improves cDNA integrity
• Efficient performance with difficult templates and GC-rich RNA

Convenient Ready-to-Use Master Mix Format

• Simplifies workflow with minimal pipetting
• Reduces variability and improves reproducibility
• Includes No-RT control for accurate experimental validation

Exceptional Performance with Challenging Samples

• Compatible with low-input RNA samples
• Tolerates inhibitors and partially degraded RNA
• Suitable for clinical, FFPE, and environmental samples

Applications

• RT-qPCR gene expression analysis
• First-strand cDNA synthesis
• Quantitative real-time PCR workflows
• RNA sequencing (RNA-seq) library preparation
• Biomarker discovery and validation
• Molecular diagnostics research
• Viral RNA detection workflows
• Functional genomics and transcriptomics

Available Models and Ordering Information

Kit Contents

SuperScript IV VILO Master Mix includes:

• SuperScript IV VILO Master Mix (5X)
• SuperScript IV VILO Master Mix No-RT Control
• Nuclease-free water

Additional components in ezDNase version:

• ezDNase enzyme for rapid genomic DNA removal

Technical Specifications

Workflow Integration

RNA Extraction → (Optional) ezDNase Treatment → Reverse Transcription with SuperScript IV VILO Master Mix → cDNA Generation → RT-qPCR or Sequencing Analysis

Invitrogen™ SuperScript™ IV VILO™ Master Mix delivers fast, high-yield, and highly sensitive cDNA synthesis for RT-qPCR and gene expression applications. With optional integrated genomic DNA removal, exceptional thermostability, and optimized workflow efficiency, this master mix provides superior reliability and performance, making it an essential tool for modern molecular biology, transcriptomics, and gene expression analysis laboratories.