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Invitrogen™ SuperScript™ IV VILO™ Master Mix
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Specifications:
| Application | PCR | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
SuperScript™ IV VILO™ (SSIV VILO) Master Mix is an advanced one-tube master mix engineered for fast, efficient, and sensitive cDNA synthesis in RT-qPCR applications. Built on the legacy of trusted VILO technology and upgraded with SuperScript IV reverse transcriptase, this master mix enables rapid reverse transcription—completing reactions in just 10 minutes.
Available with or without ezDNase enzyme, this master mix is optimized to deliver high yields of first-strand cDNA, even from low-purity or low-input RNA samples, and features an optional genomic DNA removal step for simplified workflows and greater accuracy in downstream qPCR.
Key Features
⚡ Fast & Convenient
- Complete RT reactions in just 10 minutes
- One-tube format minimizes pipetting and variability
- Optional ezDNase enables 2-minute gDNA removal
🧬 High Sensitivity & Yield
- Reduces Ct values by 2+ cycles vs. conventional kits
- Enables robust reverse transcription from low input or degraded RNA
- Produces long cDNA products—up to 10 kb
🧪 Optimized for RT-qPCR
- Contains reduced RNase H activity, improving yield and integrity
- Highly processive and thermostable SuperScript IV enzyme
- Efficient with GC-rich and inhibitor-containing RNA
🔬 Superior Compatibility
- Compatible with all downstream SYBR™ or probe-based qPCR reagents
- Ideal for gene expression profiling, miRNA quantification, and transcriptome analysis
Use Cases
✅ Gene Expression Analysis (RT-qPCR)
- Quantitative mRNA profiling with minimal background from contaminating DNA
✅ Low-Input or Challenging Samples
- Reliable performance with degraded or inhibitor-rich RNA samples (e.g., FFPE tissue)
✅ High-Throughput & Time-Sensitive Workflows
- 10-minute RT step and one-tube format reduces hands-on time
✅ gDNA-Sensitive Workflows (with ezDNase)
- Ideal for eliminating false positives in workflows that demand high specificity
What’s Included
| Component | Volume |
|---|---|
| SuperScript IV VILO Master Mix | 200 μL |
| SSIV VILO Master Mix 'No RT' Control | 200 μL |
| Nuclease-Free Water | 1.25 mL |
With ezDNase kits, additional ezDNase Enzyme and Buffer are provided.
Specifications
| Attribute | Details |
|---|---|
| Reaction Time | 10 minutes |
| Optimal Temp. | 50°C |
| cDNA Product Size | Up to 10 kb |
| Enzyme | SuperScript IV Reverse Transcriptase |
| Ribonuclease H Activity | Reduced |
| Reagent Format | Master Mix (5X) |
| Sample Type | Total RNA |
| Final Product | First-Strand cDNA |
| Compatible Applications | RT-qPCR, miRNA quantification, gene expression analysis |
| Unit Size | 50 or 500 reactions |
🔍 Comparison: SSIV VILO Master Mix With vs. Without ezDNase
| Feature | SSIV VILO Master Mix | SSIV VILO Master Mix + ezDNase |
|---|---|---|
| Genomic DNA Removal | ❌ Not included | ✅ 2-minute pre-RT gDNA removal step |
| Workflow Duration | 10–15 minutes | ~12–17 minutes (including DNase step) |
| Additional Enzyme | ❌ | ✅ Includes ezDNase enzyme and buffer |
| Sample Purity Control | Requires separate DNase treatment | Built-in DNase step simplifies workflow |
| Best For | Standard high-quality RNA samples | RNA samples with potential gDNA contamination |
Why Choose SuperScript™ IV VILO™ Master Mix?
- ✅ Fastest RT reaction on the market
- ✅ High efficiency and reproducibility across challenging RNA types
- ✅ Simplified RT-qPCR workflow in a single tube
- ✅ Accurate gene expression results even with minimal RNA input
- ✅ Optional gDNA removal step for greater flexibility
For Research Use Only. Not for use in diagnostic procedures.
- Pack Size: 50 Rxns Kit only 500 Rxns Kit only 50 Rxns Kit with ezDnase 500 Rxns Kit with ezDnase