Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)

Invitrogen™ M-MLV Reverse Transcriptase is a recombinant DNA polymerase derived from the Moloney Murine Leukemia Virus. It is engineered to synthesize complementary DNA (cDNA) from RNA templates, single-stranded DNA, or RNA:DNA hybrids. Due to its low intrinsic RNase H activity and absence of DNA endonuclease activity, it is ideal for applications requiring full-length cDNA synthesis, making it a trusted choice in reverse transcription protocols.

Key Features

• High Yield and Processivity: Synthesizes first-strand cDNA up to 7 kb in length.
• Low RNase H Activity: Minimizes RNA degradation during reverse transcription.
• Excellent Thermostability: Optimal activity at 37°C.
• Versatile Applications: Suitable for:
  • First-strand cDNA synthesis
  • RT-PCR and qRT-PCR
  • cDNA library construction
  • Primer extension assays
  • Double-stranded DNA sequencing

Enzyme Properties

• Concentration: 200 U/μL
• Activity Definition: One unit incorporates 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)·oligo(dT)25 as the template-primer.
• Source: Recombinantly expressed in E. coli carrying the pol gene of M-MLV.

Reaction Conditions

• Buffer Composition (per 50 µL):
  • 50 mM Tris-HCl, pH 8.3
  • 40 mM KCl
  • 6 mM MgCl₂
  • 1 mM DTT
  • 0.5 mM [³H]dTTP
  • 0.1 mM poly(A), 0.1 mM oligo(dT)₂₅
  • 0.1 mg/mL BSA
• Incubation: 50 minutes at 37°C

Kit Components

• M-MLV RT, (200 U/µL)
• 5X First Strand Buffer
• DTT, (100 mM)

Storage & Shipping

• Storage Temperature: –20°C
• Shipping Condition: Wet Ice
• Stability: Guaranteed when stored properly

Ordering Options

Invitrogen™ M-MLV Reverse Transcriptase is a robust and efficient enzyme for generating high-quality cDNA, essential in a wide range of molecular biology applications. Its reliable performance, low RNase H activity, and high yield make it a trusted reagent for research laboratories working with RNA-based protocols.