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Invitrogen™ LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation
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Specifications:
| Application | Flow Cytometry, Cell Analysis | ||
| Storage Temperature | -20°C | ||
| Product Type | Assay Kits | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Robust Viability Dye for Flow Cytometry — Fixation-Compatible and Violet Laser Excitable
The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit enables reliable discrimination of live and dead cells prior to formaldehyde fixation and permeabilization in flow cytometry workflows. Optimized for use with a 405 nm violet laser, this amine-reactive dye covalently binds to free amines on the cell surface and intracellularly, enabling a clear separation of live vs. dead cell populations—even after fixation.
Supplied in stable, freeze-dried vials, this fixable dye preserves staining intensity post-fixation, making it ideal for intracellular antibody staining and applications that require biohazard inactivation.
Key Features and Benefits
-
Fixable and Formaldehyde-Compatible
Covalently binds to cellular amines, maintaining viability staining after fixation/permeabilization. -
Excitation at 405 nm
Designed for use on violet-laser-equipped flow cytometers, with peak emission at ~512 nm. -
High Discrimination Power
Delivers a >50-fold difference in fluorescence between live and dead cells for clean gating. -
Stable and Convenient Format
Lyophilized vials maintain reagent stability and performance over time. Includes DMSO for reconstitution. -
Low Spectral Overlap
Minimal compensation required with other fluorochromes—ideal for multicolor flow cytometry panels.
How It Works
- Dead cells with compromised membranes take up the dye, resulting in high fluorescence intensity.
- Live cells with intact membranes label only surface amines, resulting in lower intensity.
- The result: a clear separation of live vs. dead cells, even after fixation and staining for intracellular targets.
Applications
- Live/dead discrimination in flow cytometry
- Viability gating for fixed/permeabilized samples
- Intracellular cytokine and transcription factor analysis
- Biohazard inactivation prior to analysis
- High-dimensional immune profiling
Technical Specifications
| Property | Details |
|---|---|
| Product Name | LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit |
| Catalog Numbers | L34965 (80 assays), L34957 (200 assays), L34966 (400 assays) |
| Excitation / Emission | 405 nm / ~512–526 nm |
| Color | Aqua |
| Form | Lyophilized dye + 500 µL DMSO |
| Format | Tube(s) |
| Quantity | 200 assays (L34957) |
| Detection Method | Fluorescence |
| Cell Type | Eukaryotic cells |
| Reagent Type | Amine-reactive viability stain |
| Cell Permeability | Impermeant (live-cell exclusion only) |
| Solubility | DMSO (Dimethylsulfoxide) |
| Storage Conditions | Store at –20°C, protect from light |
| Shipping Conditions | Room temperature |
| Product Line | LIVE/DEAD™ |
| Unit Size | Each (200 assays) |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Kit Contents
- 5 vials of LIVE/DEAD™ Fixable Aqua dye (lyophilized)
- 500 µL DMSO for dye reconstitution
The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is a robust, reliable solution for viability gating in flow cytometry, especially in protocols that require fixation and intracellular staining. Its compatibility with the 405 nm violet laser and minimal spectral overlap makes it ideal for multiparametric immune profiling, while its stable format ensures consistent performance across experiments.
- Pack Size: for 80 Assays for 400 Assays for 200 Assays