Invitrogen™ FIX & PERM™ Cell Permeabilization Kit

The Invitrogen™ FIX & PERM™ Cell Permeabilization Kit is a robust and versatile solution designed for flow cytometry applications. It enables efficient fixation and permeabilization of cells in suspension, allowing access to intracellular antigens without compromising scatter characteristics. This kit is ideal for simultaneous detection of intracellular and surface markers, supporting immunophenotyping, intracellular staining, and multicolor flow cytometry panels.

Key Features:

1. Efficient Fixation and Permeabilization:
   • Enables antibody access to intracellular targets while preserving cell morphology and scatter patterns.
2. Flexible Application:
   • Compatible with a wide range of biological samples, including cell lines, whole blood, and bone marrow.
3. Reduced Background:
   • Specially formulated to minimize background staining, improving signal clarity for intracellular staining.
4. Simultaneous Staining:
   • Allows for simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step, simplifying workflows.
5. Morphological Integrity:
   • Leaves the scatter characteristics of cells intact, ensuring accurate gating in flow cytometry.

Kit Contents:

1. Fixation Medium (Medium A):
   • Used to fix cells while maintaining their structural integrity.
2. Permeabilization Medium (Medium B):
   • Enables access to intracellular markers for antibody binding.

Specifications:

Applications:

1. Flow Cytometry:
   • Enables fixation and permeabilization of cells for intracellular staining of cytokines, transcription factors, and other antigens.
2. Immunophenotyping:
   • Allows simultaneous detection of intracellular and surface markers in diverse cell populations.
3. Intracellular Cytokine Staining:
   • Ideal for detecting cytokines such as IFN-γ, IL-2, and IL-17A in immune cell research.
4. Bone Marrow and Whole Blood Analysis:
   • Validated for use with challenging biological samples like whole blood and bone marrow.
5. Multicolor Staining Panels:
   • Supports complex staining protocols with minimal impact on fluorochrome-labeled antibody performance.

Benefits:

1. Preserves Cell Morphology:
   • Maintains cell integrity and scatter characteristics for accurate gating and analysis.
2. Enhanced Signal Clarity:
   • Reduces background staining, improving signal-to-noise ratio.
3. Simplifies Protocols:
   • Simultaneous staining of intracellular and surface markers streamlines workflows.
4. Validated for Multiple Sample Types:
   • Suitable for a variety of biological samples, providing versatility for diverse research needs.

How It Works:

1. Fixation Step:
   • Incubate cells with Fixation Medium (Medium A) for 15 minutes at room temperature.
2. Wash Step:
   • Wash cells to remove excess fixation medium.
3. Permeabilization and Staining:
   • Incubate cells with Permeabilization Medium (Medium B) and fluorochrome-labeled antibody for intracellular staining.

Storage and Handling:

• Store the kit at room temperature.
• Protect reagents from extreme temperatures to maintain their efficacy.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.