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NANOPORE Technologies Flow Cell Wash Kit
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Reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.
Specifications:
| Application | Next Generation Sequencing | ||
| Storage Temperature | -20°C | ||
| Product Type | Sequencing Reagents | Forms | Liquid |
| Product Brand | Oxford Nanopore Technology | ||
| Product Grade | Molecular Biology | ||
The Flow Cell Wash Kit is an essential reagent for maintaining and reusing Oxford Nanopore Technologies flow cells. It ensures efficient removal of residual library material, rejuvenates unavailable pores, and enhances throughput for sequencing workflows. Compatible with all Oxford Nanopore DNA sequencing kits and devices, the kit supports MinION, GridION, and PromethION systems.
Key Features and Benefits
- Enhanced Throughput:
- Recovers pores from the "unavailable" state to the "single pore" state, doubling sequencing output over the lifetime of a flow cell.
- Enables reloading of washed flow cells with fresh or the same library for additional data acquisition.
- Effective Cleanup:
- Removes residual nucleic acids and libraries with minimal contamination (<0.1%) for successive runs.
- Nuclease is inactivated after the wash, ensuring no impact on subsequent runs.
- Simple Workflow:
- Two-step washing procedure with user-friendly reagents.
- Preparation time of approximately 60 minutes.
- Flexible Usage:
- Compatible with all Oxford Nanopore DNA sequencing kits.
- Supports MinION Mk1B, Mk1C, GridION, and PromethION devices.
- Optimized for Real-Time Sequencing:
- Allows users to sequence until sufficient data is collected and reload the flow cell as needed.
- Provides reagents for six washes per kit.
Specifications
| Feature | Details |
|---|---|
| Preparation Time | 60 minutes |
| Reactions per Kit | 6 |
| Stability | Shipped at 2–8°C; long-term storage at -20°C |
| Shelf Life | 3 months from receipt |
| Compatibility | All Oxford Nanopore DNA sequencing kits |
| Devices Supported | MinION Mk1B, Mk1C, GridION, PromethION |
| Flow Cell Support | FLO-MIN106D, FLO-MIN114, FLO-PRO002, FLO-PRO114M |
| Reagents in Kit | Wash Mix (15 µL), Diluent (2 x 1,300 µL), Storage Buffer (2 x 1,600 µL) |
| Nuclease | Removes library nucleic acids effectively |
| Post-Wash Contamination | <0.1% of previous sample |
Applications
- Flow Cell Maintenance:
- Removes residual nucleic acids to restore flow cell performance.
- Enhanced Sequencing Output:
- Reverts unavailable pores to single pore state for increased throughput.
- Sequential Sample Loading:
- Enables loading of different libraries or samples sequentially.
- Cost-Effective Reusability:
- Maximizes the lifespan of expensive flow cells.
Protocols
- Follow the Flow Cell Wash Kit (EXP-WSH004 or EXP-WSH004-XL) protocol for effective cleaning.
- Use Sequencing Auxiliary Vials Expansion (EXP-AUX001 or EXP-AUX110) for reloading the same library post-wash.
Shipping and Logistics
- Shipped with flow cells in temperature-controlled packaging.
- Delivery schedules tailored to regions to ensure timely arrival.
- Environmentally friendly shipping materials.
Why Choose the Flow Cell Wash Kit?
- Reusability: Extends the operational life of flow cells, reducing costs.
- Reliability: Ensures consistent performance for successive sequencing runs.
- Flexibility: Supports various Oxford Nanopore sequencing devices and kits.
This kit is ideal for researchers aiming to optimize sequencing output, save costs, and maintain high-quality data in their workflows.