Invitrogen™ Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit, 96 Tests

Quantitative colorimetric detection of total antioxidant capacity in biological samples

The Invitrogen™ Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit provides a sensitive, reproducible, and rapid method for quantifying the overall antioxidant capacity of a wide range of biological samples, including serum, plasma, whole blood, tissues, cells, and culture supernatants.

T-AOC reflects the combined effect of enzymatic and non-enzymatic antioxidants in counteracting oxidative stress by neutralizing reactive oxygen species (ROS) and other free radicals. This assay measures the ability of antioxidants in the sample to reduce ferric ions (Fe³⁺) to ferrous ions (Fe²⁺), which then form a stable complex with a phenanthroline compound. The intensity of the resulting color, measured at 520 nm, is directly proportional to the total antioxidant capacity of the sample.

Key Features

• High Sensitivity – Detects as low as 0.62 U/mL
• Wide Measurement Range – 0.62–190.43 U/mL
• Simple & Fast Workflow – ~50 minutes total assay time
• Reliable & Reproducible – Low intra-assay (4.8%) and inter-assay (5.6%) CVs
• Versatile Sample Compatibility – Works with multiple sample types including cell culture supernatants and biological fluids
• Direct & Quantitative – Colorimetric readout at 520 nm for accurate results
• The Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit Calculator is typically used to convert your raw absorbance readings (at 520 nm) into antioxidant capacity units (U/mL) based on your standard curve.
  

Applications

• Oxidative Stress Research – Assess total antioxidant status in disease models
• Nutritional & Food Science – Evaluate antioxidant potential of dietary supplements or food extracts
• Pharmaceutical Development – Screen antioxidant drug candidates
• Aging Research – Study antioxidant defense systems in age-related processes
• Clinical & Biomedical Research – Investigate antioxidant imbalance in health and disease

Specifications

The Invitrogen™ Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit is an essential tool for oxidative stress, nutritional, and biomedical research, allowing scientists to quantify overall antioxidant defense quickly and accurately. Its broad sample compatibility, high sensitivity, and simple workflow make it ideal for routine and high-throughput antioxidant screening applications.